转基因植物功能效率的评价ppt.ppt

转基因植物功能效率的评价ppt
第1页，共15页，编辑于2022年，星期三
转基因及转基因植物
关于转基因植物功能效率的理解
功能效率评价实验技术路线
经典实验文献解读
关于转基因植物的思考
目 录
第2页，nta event Bt11) and their non-Bt, isoline hybrids. Potted plants were artificially infested with 1st instar larvae. Percentage larval survival and mean larval mass were recorded over maize of both events was shown to be highly toxic toS. calamistis. No larvae survived longer than 9 days on plants of either of the Bt . calamistisis stenophagous and occurs in mixed populations with other borer species, with which it shares several parasitoid species in Africa. The ecological impact of local extinction ofS. calamistis caused by this highly effective transgenic event is therefore not
expected to be great.
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※Background
Bt from the bacteriumBacillus thuringiensis(Bt)can produce insecticidal proteins .These proteins can kill Busseola fusca andChilo partellus
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and methods
Plant:
DKC 78-15B(MON810) and CRN 3505
NK Mayor B(Bt11) and Brasco
Larvae:
Sesamia calamistis
Methods:
Ⅰ.Greenhouse
Ⅱ.The experimental layout was a completely randomized design.
Ⅲ. plants of each hybrid were inoculated with 10 neonate larvae. transfer 1st instar larvae onto plants between the stem and the last unfolded leaf sheath of the plant .
Ⅳ.The number of surviving larvae and the mean mass of larvae per
plant were determined at weekly intervals. Nine randomly selected
plants of each maize hybrid were dissected at 3, 6, 9, 12, 20, 33 and
42 days after inoculation.
Ⅴ.Repeated measures ANOVA
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5. 关于转基因植物的思考

文献所展示的方法可以应用的范围很广泛，比如抗草甘膦的转基因大豆功能效率的评价，以及实验室部分功能基因RNAi效果的分析，都可以利用这种方法。

转基因植物除了供科研单位研究以外大多用于商业用途，例如孟山都、先正达、先锋，都是知名的转基因品种研制和生产企业，对转基因植物功能效率进行评价可以通过实验室检验直观的了解这些目的转基因植物和商业品种的功能效率，不仅可以对这些材料的使用具有指导作用，更能对市场上的转基因品种进行必要的检验。
第12页，共15页，编辑于2022年，星期三