英文单隔室模型(实验) 药大.ppt

Simulation of One-Compartment Model
China Pharmaceutical University
Purpose
Principle
Procedure
Questions
Results
Simulation of One-Compartment Model
China Pharmaceutical University
Purpose
Principle
Procedure
Questions
Results
To be familiar with the basic procedures in the simulation of the one-compartment model.
To master the estimation methods for pharmacokinetics parameters using data of simulation experiment
Purpose
Principle
a one-compartment model




Drug concentration in the plasma
The rate method of urinary excretion
X0
iv
k
X（t), V
Following rapid intravenous injection of a drug that distributes in the body according to a one-compartment model and is eliminated by apparent first-order kinetics

the time course of plasma concentration of drug is given by
Drug concentration in the plasma
Drug concentration in the plasma
slop：-k
intercept ：lnC0
The rate method of urinary excretion
X0
iv
ke
X（t), V
Xu
Xnr
knr
log(∆Xu/ ∆t)—t中
作图得k、ke
The excretion rate of unchanged drug, dXu/dt, can be defined as :
Urinary excretion method——sigma-minus method
k= -斜率值
ka is obtained from the
intercept of this curve
Procedure
Materials
Instruments

Simulation of One-
Compartment Model
Procedures
the simulated device
250ml of water
take 10ml of water from the flask
add 10ml of % phenol red
into the flask
at 10 min intervals
take ml of water
as the observed
“plasma” sample
collect the solution effused from the branch B as the observed “urinary” sample
Determination of phenol red
Determination of phenol red ：
of “plasma” sample or “urine” sample
+ λ=555nm，A
5ml M NaOH
Preparation of standard curve :
A series of standard solutions (5 - 30μg/ml) were prepar