1 慢性哮喘小鼠 EGF R 的表达及地塞米松对其的影响【关键词】哮喘 Expression of epidermal growth factor receptor and its ligands in chronic asthma mice and the influence of dexamethasone 【 Abstract 】 AIM: To investigate the effect of epidermal growth factor receptor (EGFR) system on the asthmatic airway : The chronic asthma mice model was established by ovalbumin (OVA) immunization. Mice were injected intraperitoneally with dexamethasone of2 mg/kg 1h before the sensitization and sacrificed by cervical dislocation 24 h after the last challenge. Conventional pathological slides were also observed to study the pulmonary inflammation and the thickness of airway wall and airway smooth muscles. The concentration of TGF α in bronchoalveolar lavage fluid (BALF) was detected by ELISA. RTPCR was conducted to detect the mRNA expression of EGFR in the lungs. Pulmonary EGFR 2 protEin was observed by immunohistochemical staining and Western blot analysis. RESULTS: In chronic asthma mice, inflammatory cell infiltration around bronchi and vessels could been seen on slides. The airway wall and the airway smooth muscles thickened and TGF α in BALF increased. The expression of EGFR mRNA and protEIn in the lungs increased (). Dexamethasone injection before sensitization decreased TGF α in BALF and the expression of phosphoEGF receptor protein in the lung alleviated the pulmonary inflammation and inhibited the thickening of airway wall and airway smooth muscle (). CONCLUSION: Airway remodeling occurs in the chronic mice asthma model. Early dexamethasone injection can inhibit the expression and activation of EGFR system, decrease the TGF α concentration in BALF and prevent the airway remodeling. 【 Keywords 】 asthma; airway remodeling; receptor, epidermal growth factor; transforming growth factor alpha; dexamethasone 【摘要】目的: 探讨表皮生长因子受体( EGFR )在慢性哮喘气道重建中的作用. 方法: 建立小鼠慢性哮喘模 3 型, 致敏前 1h 气道内滴入地塞米松 2 mg/kg , 最后一次激发 24h 后处死动物,常规病理切片观察小鼠肺内炎症情况、气管壁厚度及气道平滑肌厚度, ELISA 法测定肺泡灌洗液中 TGF α的浓度, RTPCR 观察小鼠肺部 E
