电针联合西乐葆缓解大鼠胫骨癌痛.doc

1 电针联合西乐葆缓解大鼠胫骨癌痛作者:毛应启梁, 任典寰, 米文丽, 刘琼, 王彦青【摘要】目的: 建立大鼠胫骨癌痛模型, 观察电针和西乐葆对大鼠胫骨癌痛的缓解作用。方法: 采用经皮穿刺技术将大鼠乳腺癌细胞 Walker 256 接种至雌性 Wista r 大鼠胫骨骨髓腔内, 建立大鼠胫骨癌痛模型。大鼠造模后, 采用电针和西乐葆灌胃治疗, 观察其对大鼠骨肿瘤引起的机械性痛觉超敏的影响。结果: 模型组大鼠后肢逐渐产生机械性痛觉超敏,接种后第 16 天胫骨近心端可见显著的肿瘤生长。单用电针或西乐葆 5 mg/(kg · d) 治疗大鼠, 与模型组相比其机械性疼痛阈值差异无统计学意义; 治疗后第22 天和第26天, 西乐葆 10 mg/(kg · d) 组大鼠后肢机械性疼痛阈值较溶剂对照组提高( ) ;治疗后第 10、 18和 23 天,电针合用西乐葆5 mg/(kg · d) 组大鼠机械性疼痛阈值高于模型组, 差异有统计学意义( ) 。结论:电针与小剂量西乐葆具有协同作用,合用可以增强对大鼠胫骨癌痛的镇痛效果。【关键词】骨癌痛电针西乐葆镇痛大鼠 2 Objective: To establish a proper experimental model of bone cancer pain in rat for acupuncture research, and observe the pain 拟 relieving effect of electroacupuncture (EA) and/or Celebrex on bone cancer pain in rats. Methods: The rat model of bone cancer pain was established by percutaneous direct puncture technique and inoculating the rat mammary gland carcinoma cells Walker 256 into tibial medullary cavity directly, and evaluated by detecting the bone tumor growth and mechanical allodynia. The effects of daily EA treatment and/or Celebrex treatment on the rat mechanical allodynia after intratibial Walker 256 inoculation was observed in this study. Results: Significant mechanical allodynia in ipsilateral hind paw and tumor growth in proximal end of tibial bone of rats in the untreated group were observed after intratibial Walker 256 inoculation. The mechanical allodynia thresholds in rats that recEived EA or5 mg/(kg · d) Celebrex treatment showed no significant difference pared with that of rats in the untreated group. However, the mechanical allodynia thresholds 3 of rats in 10 mg/(kg · d) Celebrex group showed significant increase after 22拟 and 26拟 day treatment pared with that in the methyl cellulose (MC) group. There was significant difference between rats with bined with 5 mg/(kg · d) Celebrex treatment and rats in the untreated group after 10拟, 18 拟 and 23拟 day treatment. Conclusion: EA and 5 mg/(kg · d) Celebrex have synergistic effect on pain relieving and bined use may enh