脓毒症急性肺损伤大鼠肺组织的细胞凋亡_0.doc

1 脓毒症急性肺损伤大鼠肺组织的细胞凋亡作者:徐笑益方向明鲍军明石卓顾伟忠【关键词】脱噬作用;脓毒症;肺损伤[ 摘要] 目的观察脓毒症急性肺损伤( ALI ) 大鼠肺组织的细胞凋亡。方法 24只 SPF 级 SD 大鼠采用盲肠结扎穿孔术( CLP ) 复制脓毒症模型, 分别于 CLP 后0、 12、 36和 72h 颈椎脱臼法处死大鼠,取肺组织。行肺组织 HE 染色,观察病理变化, 以透射电镜、 TUNEL 法检测细胞凋亡。结果 HE 染色可见, CLP 后 12、 36、 72h 肺泡间隔增宽,间质充血水肿,肺泡腔变窄,炎症细胞渗出。 TUNEL 法检测发现, CLP 后 12、 36、 72h 肺组织内细胞凋亡指数( AI )增加,其中 36hAI 值最高( ) 。透射电镜证实细胞凋亡的特征性改变。结论脓毒症大鼠肺组织的 AI 明显增加, 细胞凋亡可能在脓毒症 ALI 发生、发展中起着重要作用。[ 关键词] :脱噬作用;脓毒症;肺损伤[Abstract] Objective To investigate the pulmonary apoptosis 2 of acute lung injury(ALI) in sepsis. Methods 24 S-D rats were performed cecal ligation and puncture(CLP) to induce sepsis. At the time of 0, 12, 36 and 72 hours after CLP, the rats were killed. Lungs were fixed for haematoxylin and eosin(H(E) stain. Apoptosis was determined by TUNEL test (Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) and electron microscope. Results At 12, 36, 72 hours following CLP, interstitial edema occurred with massive infiltration of the inflammatory cell, and the pulmonary architecture was damaged. Electron microscope demonstrated characteristic findings of apoptosis. TUNEL test showed more positive cells at 12, 36, and 72 pared with that at0 hr. Conclusion Apoptosis may play an important role in the development of ALI. 【 Key Words 】 apoptosis; sepsis; acute lung injury 脓毒症(sepsis, 又称全身性感染) 是临床危重病患者主要的死亡原因之一, 目前尚无治疗脓毒症的有效方法。在美国每年有 75 万人发生脓毒症, 其中有