HPLC测定不同产地粉萆薢中原薯蓣皂苷薯蓣皂苷的含量.docx

HPLC法测定不同产地粉萆薢中原薯蓣皂苷和薯蓣皂苷含量
邵岩岩作者简介:邵岩岩,北京市朝阳区望京中环南路6号北京中医药大学,邮编:100102,电话:010-84738629,邮箱:sunshine4003@
,肖潇1,吴玲芳1,朱丹1,杨光辉1,张晓雪1,张兰珍通讯作者:张兰珍,北京市朝阳区望京中环南路6号北京中医药大学,邮编:100102,电话:010-84738629,邮箱:zhanglanzhen01@
(北京中医药大学中药学院,北京 100102)
摘要: 目的:建立 HPLC 法测定不同产地粉萆薢中原薯蓣皂苷和薯蓣皂苷含量。方法:采用 Agilent C18 色谱柱( mm × 150 mm,5 μm),以乙腈- 水为流动相,梯度洗脱, mL·min-1,检测波长为 208 nm,柱温30 ℃,进样体积 20 μL。结果: ~ μg(r = 0. 9996)范围内线性关系良好, %(RSD = %); ~ μg(r = )范围内线性关系良好, %(RSD = %)。 ~ %之间, 薯蓣皂苷的含量在 ~ %之间。结论:该方法操作简便,定量准确,重现性好,可为粉萆薢药材质量控制提供方法。
关键词: 粉萆薢;原薯蓣皂苷;薯蓣皂苷;不同产地;含量测定;高效液相色谱
基金资助: 北京中医药大学科研创新团队项目(-CXTRD-12)
Determination of protodioscin and diosgenin in
Dioscorea hypoglauca from different habitats by HPLC
SHAO Yan-yan1, XIAO Xiao1, WU Ling-fang1, ZHU Dan1, YANG Guang-hui1,
ZHANG Xiao-xue1, ZHANG Lan-zhen1
(1School of Chinese Materica Medica, Beiing University of Chinese Medicine, Beijing 100102, China)
Abstract: Objective: To establish an HPLC method for the content determination of
protodioscin and diosgenin in Dioscorea hypoglauca pare the contents from different habitats. Methods: The chromatographic separation was performed on an Agilent C18 column ( mm × 150 mm, 5 μm); with mobile phase of acetonitrile – water solution with gradient elution at the flow rate of mL·mi