用毛细管电泳分离蛋白质.pdf

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Anal Chim Acta. Author manuscript; available in PMC 2013 January 4.
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Anal Chim Acta. 2012 January 4; 709: 21–31. doi:..
Protein Separation by Capillary Gel Electrophoresis: A Review
Zaifang Zhu, Joann J. Lu, and Shaorong Liu*
Department of Chemistry and Biochemistry, University of Oklahoma, Norman, OK 73019
Abstract
Capillary gel electrophoresis (CGE) has been used for protein separation for more than two
decades. Due to the technology advancement, current CGE methods are ing more and more
robust and reliable for protein analysis, and some of the methods have been routinely used for the
analysis of protein-based pharmaceuticals and quality controls. In light of this progress, we survey
147 papers related to CGE separations of proteins and present an overview of this technology. We
first introduce briefly the early development of CGE. We then review the methodology, in which
we specifically describe the matrices, coatings, and detection strategies used in CGE. CGE using
microfabricated channels and incorporation of CGE with two-dimensional protein separations are
also discussed in this section. We finally present a few representative applications of CGE for
separating proteins in real-world samples.
Keywords
Capillary gel electrophoresis; Proteins; Capillary electrophoresis; Capillary sieving electrophoresis
1. Introduction
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE, see Table I for a
list of acronyms used in this paper) has been used for size-based separations of proteins for
over four decades [1, 2], and it is still the workhorse for protein separations and analyses in
most biological research laboratories. The basic procedures of this method include: 1)
preparing a gel and assembling the gel apparatus, 2) mixing a protein sa