壳聚糖基角膜细胞载体的制备及其细胞相容性.pdf

生物工程学报 Chin J Biotech 2008, August 25; 24(8): 1381-1386
. Chinese Journal of Biotechnology ISSN 1000-3061
******@im. © 2008 Institute of Microbiology, CAS & CSM, All rights reserved

研究报告
壳聚糖基角膜细胞载体的制备及其细胞相容性
高兴爽, 刘万顺, 韩宝芹, 位晓娟
中国海洋大学海洋生命学院, 青岛 266003
摘要: 为探讨羟丙基壳聚糖基共混膜作为组织工程技术中角膜细胞培养载体的可行性, 分别制备了羟丙基壳聚糖/硫
酸软骨素、羟丙基壳聚糖/明胶/硫酸软骨素以及羟丙基壳聚糖/氧化透明质酸/硫酸软骨素三种共混膜。测定其透光率、
含水量和蛋白吸附性能; 在共混膜上培养兔角膜上皮细胞, 通过观察角膜上皮细胞在不同载体膜上的生长状态、贴附情
况, 测定细胞活性以及上清液中乳酸脱氢酶的活性, 研究三种壳聚糖基载体膜片与角膜上皮细胞的相容性。膜片理化性
质测定结果表明三种共混膜片具有良好的透明度, 适宜的含水量和较强的蛋白吸附性能; 细胞相容性实验结果表明羟
丙基壳聚糖/明胶/硫酸软骨素共混膜对细胞的损伤最小, 有利于细胞在膜上的贴附和生长, 表现出良好的细胞相容性,
有望作为角膜细胞载体体外构建组织工程化角膜。
关键词: 壳聚糖, 载体, 角膜上皮细胞, 细胞相容性
Preparation and patibility of Chitosan-based
Carriers of Corneal Cells
Xingshuang Gao, Wanshun Liu, Baoqin Han, and Xiaojuan Wei
College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
Abstract: To study the possibility of using hydroxypropyl chitosan-based blend membranes as carriers of corneal cells in tissue
engineering, we prepared three kinds of blend membranes labeled hydroxypropyl chitosan/chondroitin sulfate, hydroxypropyl
chitosan/gelatin/chondroitin sulfate and hydroxypropyl chitosan/oxidized hyaluronic acid/chondroitin sulfate. The transparency,
water content and ability of protein adsorption of the blend membranes were measured. To evaluate the patibility of the
blend memb