DHA含量测定-英文版.doc

Weishi Biological Technology Co., Ltd. Zhejiang
The determination of the content of the DHA
Principle
Fatty acids in oils and fats unter the boron (tri)fluoride and methanol and sodium hydroxide , as a fatty acid methyl ester, the fatty acid methyl ester’s respondence on gas chromatograph hydrogen flame ionization detector linearly related with its existent amount. The content of variety of fatty acids can be calculated by the content of a variety of fatty acid methyl ester on chromatography.
Application
Applicable to the determination of conventional content of DHA oil or powder.
Sample volume
Sample about 2g.
Apparatus
Volumetric flask of 25ml
Transfer pipet of 10ml
Gas chromatography equipment and operating conditions
Column Oven
Initial temperature:90℃(开)
Maximum temperature: 300℃
The initial time: min
Balance time: min
Temperature gradient
# Career The final temperature The final time
Operating temperature after:50℃
1 240
Operating time after:
2 250
Operating time: min
3 (关)
Forward injection port (streaming / non-streaming) mode: streaming, 1:100 ,initial temperature:250℃(开)
chromatographic column
Capillary column,specification:DB-23 122-2332
Maximum temperature:300 Length: Diameter: Film thickness:
Detector (FID)
Temperature :300℃
flow rate of hydrogen
30ml/m