免疫磁珠说明书.doc

Contents
1. Description
Principle of the MACS® Separation
Background information
Applications
Reagent and instrument requirements试剂和仪器的要求
2. Protocol
Sample preparation样品制备
ic labeling磁性标记
ic separation磁性分离
3. Example of a separation using the CD133 MicroBead Kit
4. References
1. Description
Components 2 mL CD133 MicroBeads, human:MicroBeads conjugated to monoclonal antihuman
CD133 antibodies (isotype: mouse IgG1,clone AC133).2 mL FcR Blocking Reagent, human
Specificity CD133 antigen, epitope (CD133/1) For 2亊10⁹ total cells, up to 100 format CD133 MicroBeads are supplied in buffercontaining stabilizer and % sodium Store protected from light at 2−8 °C. Do not freeze. The expiration date is indicated on the vial label.
Principle of the MACS® Separation
First, the CD133+ cells are ically labeled with CD133
MicroBeads. Then, the cell suspension is loaded onto a MACS®
Column, which is placed in the ic field of a MACS Separator.
The ically labeled CD133+ cells are retained within the
column. The unlabeled cells run through; this cell fraction is
thus depleted of CD133+ cells. After removing the column from
the ic field, the ically retained CD133+ cells can be
eluted as the positively selected cell fraction. To increase the purity,
the positively selected cell fraction containing t