氢氯噻嗪的利尿与抗利尿作用致大鼠尿量及组织含水量的变化.doc

氢氯噻嗪的利尿与抗利尿作用致大鼠尿量及组织含水量的变化.doc氢氯噻嗪的利尿与抗利尿作用致大鼠尿量及组织含水量的变化
:云天佑,王杨,蔡晓东,谢富生,刘嫱
【摘要】目的:探讨药物氢氯噻嗪对健康机体的利尿作用与抗利尿作用间的规律关系。方法:对应用氢氯噻嗪健康大鼠组和无药对照鼠组,分别采用自由饮水、少量清水灌胃(4h:125mL·kg-1)、和较大量清水灌胃(4h:175mL·kg-1)及超量清水灌胃(4h:260mL·kg-1)的“清水利尿”条件时,观察给水量不同条件时大鼠6h尿量的变化,及超量水灌胃后大鼠血浆渗透压、脑组织含水量及肾组织病理切片水肿情况。结果:自由饮水和少量水灌胃条件时,用药鼠的6h尿量多于未用药鼠(P<);在较多水灌胃和超量水灌胃条件时,用药鼠的6h尿量少于未用药鼠(P<);在超量水灌胃(4h:260mL·kg-1)后,用药鼠血浆渗透压比未用药鼠低,而用药鼠脑组织含水量比未用药组鼠多(P<),用药组鼠肾组织病理切片出现明显的水肿,未用药组鼠无明显水肿。结论:氢氯噻嗪本身具有利尿作用与抗利尿作用并存,即当尿量不多时显现出利尿效应,当尿量过多(如水利尿)时显现出抗利尿效应;且若进入机体水量过多,该药可致水中毒。
【关键词】氢氯噻嗪;抗利尿作用;水中毒;血浆渗透压;脑组织含水量
[ABSTRACT] Objective: To explore diuretic and relative antidiuretic effect of hydrochlorothiazide on various ly divided into hydrochlorothiazide administration group (group A) and nonadministration group (group B). Tetabolic cage, and six cages e (intragastric administration L/kg in 4hrs, ), medium volume (175 mL/kg in 4 hrs, ) and excessive L/kg in 4 hrs, ) ployed to each group( group A and B, respectively). In first round, quantities of urine volume in the first 6 hrs (Vol6), plasma osmolality (Posmol) and cerebral tissue content of inistration for 3 days (or placebo) in groups. Controlled ployed in the second and the third round in group A and B, Vol6 and CH2O es in the first 6 hrs . and rats, but significantly loa osmolality level in group A rats, a changes could be found in group A rats. Con