响应面分析法优化茶树菇多糖的提取工艺研究.pdf

2008年 9月
第 29卷第 9期≤鏊蠢麓科学研究
— 24
响应面分析法优化茶树菇多糖的提取工艺研究
李效贤,王芳芳,余陈欢,熊耀康
(浙江中医药大学药学院,浙江杭州 310053)
摘要:研究微波法提取茶树菇多糖的优化提取工艺。以茶树菇粗多糖含量为考察指标,采用响应面分析法优化微
波提取工艺,考察微波辐射频率、微波时间、料液比等 3个因素对茶树菇多糖提取率的影响。茶树菇粗多糖优化提取
工艺为:44倍量水,在微波辐射频率960MHz下微 1 波min。该 6 工艺稳定可行。
关键词:茶树菇;多糖;响应面分析法
0PTIMIZATION0F THE EXTRACTION TECHNOLOGY OF SOLUBLE HARIDES
FROMGA DC】,口ECYL VD CA BYRESP0NSIVE SURFACES ANALYSIS
LIXiao—xian,WANG Fang-~ng,YU Chen-huan,XIONG Yao—kang
(Acadamy of Pharmacy,Zhejiang Chinese Medical University,Hangzhou 3 10053,Zhejiang,China) 3 University,Hangzhou Medical Pharmacy,Zhejiang Chinese (Acadamy of
Abstract:The extractiontechnology solubleof harides fromgrocybe A optimum ex—
tracted parameters were confirmedby responsive surfaces analysis,undervarious conditionsthefor maximum
yield and content of optimum conditions of extracting harides from Agrocybe
cylindraceawere determined follow:frequency as microwave of radiation 960 1 MHz,microwave radiationtime 6
minmaterialsolution toand wasof ratio optimized technology was andstable highly efficient.
Keywords:A grocybe cylindracea;harides;responsive surfaces analysis
茶树菇Fr.)Rcylindracea( Maire又 grocybe A 解,冷却,加水稀释至mL,储于棕色瓶 000 1 中,备用。
名茶薪菇、杨树菇、柱状田头菇等,为层菌纲粪锈伞科$22pc可见分光光度计:上海棱光技术有限公司;
田菌属真菌,被人们称为“菇中珍品”。多糖是茶树菇的 ME一6500微波炉:herald。
重要化学成分之一,具有抗肿瘤,免疫调节等作用_l】。据
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