郁乐疏治疗抑郁症的实验分析.docx

成都中医药大学硕士学位论文
缩略词表
DEP Depression 抑郁症
5——Hydroxyptamine 5一羟色胺
NE Norepinephrine 去甲肾上腺素
DA Dopamine 多巴胺
SSRI Selective serotonin reuptake inhibitor 选择性5-HT再摄取抑制剂 MHPG 3-methoxy-·4-hydroxyphenyl glycol AC adenylate cyclase 腺苷酸环化酶
BDNF brain—derived neurotrophic factor 脑源性神经营养因子
MAO monoamine oxidase 单胺氧化酶
GC glucocorticoids 糖皮质激素
CREB cAMPresponse element binding protein cAMP反应元件结合蛋白 CMUS chronic mild unpredictable stress 慢性未预知温和刺激模型 5-hydroxytryptophan
CRH corticotropin releasing hormone 促肾上腺皮质释放激素 ATCll adrenocorticotropic hormone 促肾上腺皮质激素 sIL厂R soluted interleukin receptor 呵溶性白介素受体
GUJ glumatic acid 谷氨酸
PVN Paraventricular nucleus 下丘脑室旁核
成都中医药大学硕士学位论文
摘要
耳的:慢性应激结合孤养制备抑郁大鼠模型,观察郁乐疏对大鼠抑郁行为
。方法:采用孤养结合慢性未预知温和刺激制备抑郁大鼠模型。运用糖水消
耗量和敞箱实验评定大鼠的行为学改变,并用荧光分光度法测定大鼠脑内的单胺递质及单胺氧化酶含量,通过统计学方法比较组间差异,说明郁乐疏对抑郁大鼠的影响。
结果:模型大鼠出现了明显行为学异常,体重增长、糖水消耗量、敞箱实验评定、脑内单胺递质、MAO含量均与正常组有显著差异,郁乐疏高、中剂量组可显著提高抑郁大鼠的糖水消耗量、敞箱实验评定、脑内单胺递质含量, 降{氐MAO,其疗效与西药百忧解相当。
结论:孤养结合慢性未预知的温和刺激可制各比较理想的抑郁症模型,郁乐疏可显著改蒂抑郁模型大鼠的行为学异常、 增加脑内单胺递质含量、 降低 MAO活性,且呈~定的量效关系。郁乐疏的抗抑郁作用可能与增加脑内单胺递质含量、降f氐MAO活性有关。
关键词:大鼠行为学单胺递质单胺氧化酶郁乐疏
成都中医药大学硕士学位论文
ABSTRACT
0bjective: Set up the rat model to estimate the treatment of YuLeShu on the influence of YuLeShu on the depressive beh. avior,the monoarnine neurotransmitters and monoamine oxidase, and thr
OU曲t it to probe the anti-depressive mechanism of YuLeShu.
Methods: condition and chronic mild unpredictable stress are applied essively to set uD DEP rat rat is assessed by Suc rose solution consumption test and Open—field are used to estimate the change of interest,the leveI of spontaneous motor activities and behavior of DEP rat iS applied to detect the monoarnine neur— otransmitters( and DA)and MAO of the frontal·parietal - uating the efficiency of YuLeShu on the depressive behavioral tests and cereb— ral monoamine neurotrans