Exponential-,,treatedwith1%osmiumtetroxidefor1hatroomtemperature,centrifuged,%%osmiumtetroxideat4℃.Thecellsweredehydratedusinggradedethanol(10%,30%,50%,70%and100%),(SEM)ForSEManalysis,%(v/v)glutaraldehydefor2–-bufferedsaline(PBS)()forthreetimes(10mineach).Subsequently,ethanolconcentrationgradient(v/v)of50%,70%,80%,90%and100%,theresultedcellsampleswerefurtherdehydratedbytwomore100%,tertiarybutylalcoholmixedwithethanolinaratioof1:,,washedthreetimeswithPBS().Subsequently,thecellswerefixedwith2%glutaraldehyde()atroomtemperaturefor2handwashedagainas
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