高氧液对兔全脑缺血再灌注损伤生化指标的影响.doc

高氧液对兔全脑缺血再灌注损伤生化指标的影响
【关键词】高氧液
关键词: 高氧液;脑缺血;再灌注损伤;自由基;一氧化氮
摘要:目的探讨高氧液对兔全脑缺血再灌注损伤的保护作用. 方法 18只家兔随机分3组(n=6). kg-1 静脉推注(对照组);在再灌注时用高氧液20mL kg-1 静脉推注(治疗组);C组于实验前3d用高氧液20mL kg-1 静脉推注每日1次(预处理组).各组在缺血前、缺血20min和再灌注45min抽取颈内静脉血测定血浆一氧化氮(NO),超氧化物歧化酶(SOD)和丙二醛(MDA). 结果再灌注后B组和C组MDA含量明显低于A组(P<<),B组和C组血浆SOD活性高于A组(P<).B组和C组血清NO含量明显低于A组(P<<). 结论高氧液对神经元缺血损伤具有保护作用.

Keyword:hyperoxia solution;brain ischemia;reperfusion in-jury;free radical;nitric oxide

Abstract:AIM To investigate the protective effect of hy-peroxia solution on the cerebral ischemia 18domestic rabbits were randomly divided into three Group A the balance salt20mL kg-1 (whi
ch was replaced by hyperoxia solution in Group A)was given intra-venously to the animals after in Group C hyperoxia solution20mL kg-1 was infused intravenously ev-eryday for3d before amount of NO and MDA as well as the activity of SOD in plasma were observed at dif-ferent time points(before ischemia,after20min ischemia and after45min reperfusion).RESULTS 45min after reperfusion,the amounts of NO and MDA in Group B and Group C were lower than those in Group A(P<
d P<).But the activity of SOD in Group B were significantly higher than in Group A(P<).CONCLUSION Hyperoxia solution can miti