奈替米星的光度分析研究.doc

奈替米星的光度分析研究
【摘要】奈替米星(NTM)与曲利本蓝(TB)~,其最大吸收波长位于688nm,线性范围为0~;最大褪色波长为592nm,线性范围为0~;表观摩尔吸光系数(ε)×104(正吸收)L/(mol·cm)×104(负吸收)L/(mol·cm)。当用双波长叠加法时,×104L/(mol·cm)。本文探讨了适宜的反应条件及主要分析化学性质。该法操作简便,有较高的灵敏度和选择性,用于市售奈替米星药物中奈替米星含量的测定,结果满意。
【关键词】奈替米星; 曲利本蓝; 分光光度法
ABSTRACT In the condition of pH ~, netilmicin (NTM) reacts with trypan blue (TB) to form blue ion plexes with an obvious absorption peak and an fading peak. The maximum absorption wavelengths were located at 688nm, the linear range was 0~; The maximum fading wavelengths were located at 592nm, the linear range was 0~; Their apparent molar absorptivities (ε) part was ×104 (positive absorption) and ×104 (negative absorption ) L/(mol·cm). When the dualwavelength overlapping absorption spectrometry was used to determine ilmicin, its apparent molar absorptivities (ε) achieved ×104 L/(mol·cm). The optimum reaction conditions and the ch
ief properties of analytical chemistry were studied. The method was simple and convenient, moreover possess higher sensitivity and selectivity, it could be applied to determine the content of ilmicin with satisfactory results.
KEY ilmicin; Trypan blue; Spectrophotometry
ilmicin)是美国ScheringPlough公司开发的一种氨基糖苷类抗生素,其抗菌谱广,活性强,耳、肾毒性较其它同类药物小,已广泛用于临床。目前已报道的检测奈替米星的方法主要有微生物法[1,2]、HPLC法[3~8]、HPLC间接光度法[9,10]、薄层色谱法[