胜利原油对半滑舌鳎幼鱼肝脏谷胱甘肽过氧化物酶和谷胱甘肽转硫酶活性的影响.doc

胜利原油对半滑舌鳎幼鱼肝脏谷胱甘肽过氧化物酶和谷胱甘肽转硫酶活性的影响Vol,30,No,6306海洋环境科学第卷第期201112年月eceber2011DmIEEIETLMARNNVRONMNASCIENCE胜利原油对半滑舌鳎幼鱼肝脏谷胱甘肽过氧化物酶和谷胱甘肽转硫酶活性的影响1,211112,,,,,李传慧夏斌陈碧鹃崔毅张艳娄安刚(1,,,中国水产科学研究院黄海水产研究所农业部海洋渔业资源可持续利用重点开放实验室山东省渔业资源与生态环境重点实验室,山东青岛266071;2,中国海洋大学环境科学与工程学院,山东青岛266100):1、2、4、8、15d1d3d摘要在实验室条件下研究了不同浓度胜利原油暴露以及解除污染和后对半滑舌鳎幼鱼肝脏谷胱(GSH-PX)(GST)。GSH-PX:15d,甘肽过氧化物酶和谷胱甘肽转硫酶活性的影响结果表明曝油内各浓度组活性呈峰,,4d,3,2g/L1d(P,0,01),15d3,2g/Lmm值变化先增大后减小第时升高到最大值其中组在第就被显著诱导第除组仍(P,0,01),(P,0,05);GST0,10,2mg/L,极显著高于对照组外其它各组均恢复至对照组水平活性除和组变化不大外其,0,4、0,81,6mg/L8d,3,2mg/L2d,15dGST余各组均呈先增加后减小的趋势和组第达到最大值组第达到峰值第各组活。S-PST,GHXG性均恢复至对照组水平从污染胁迫解除后和活性的恢复情况分析原油污染对半滑舌鳎幼鱼肝脏GSH-PX,20,4mg/L,GST0,81,6mg/L。0活性影响的阈值在和之间而对活性影响的阈值在和之间半滑舌鳎幼鱼肝脏GSH-PXGST,,活性对石油污染的响应相对于更加敏感更能反映出早期污染氧化胁迫有望成为石油类污染的生物标志。物:;;;::1007-6336(2011)060827:A--关键词胜利原油半滑舌鳎谷胱甘肽过氧化物酶谷胱甘肽转硫酶中图分类号文章编号文献标识码Q33104EffectsofShenglicrudeoilonactivitiesofGSHPXandGST-inliverofCynoglossussemilaevislarva1,211112LIChuan-hui,XIABin,CHENBi-juan,CUIYi,ZHANGYan,LOUAn-gang(1,eyLaboratoryforSustainabletilizationofarineFisheriesesource,inistryofgriculture,eyLaboratoryforFisherye-sourcesKUMRMAKRandEco-environmentofShandongProvince,YellowSeaFisheriesResearchInstitute,ChineseAcademyofFisherySciences,ingdao266071,China;2,CollegeofEnvironentalScienceandEngineering,ceanniversityofChina,ingdao266100,China)QmOUQAbstract:TheeffectsonactivityofGSH-PXandGSTinjuvenileCynoglossussemilaevis,whichwasexposedtotheShenglicrudeoilattheenvironmentallevels(0,0,1,0,2,0,4,0,8,1,6and3,2mg/L)for1,2,4,8,15daysandremovedpollutionfor1,3days,weree-valuated,Theresultsshowedthatinthe15-dayoilexposureperiod,theliverS-Pactivitywasincreasedtotheaxiuonthe4thdayGHXmmmandthendecreased,TheGSH-PXactivityofgroup3,2mg/Lwasinducedsignificantly(P,0,01)onthefirstday,andtheactivityofalltheothergroupsexceptthehighestconcentrationgrouprestoredtotheoriginallevel,TheGSTactivityhadthesimilartendencywithGSH-PX,andtherewasnotthesignificantchangeintheactivityoflowerconcentrationgroups(0,1and0,2mg/L),Themaxi-mumvaluesofgroup0,4