中dl大学博士学位踣文重组五步蛇毒降纤酶的基因克隆、表达、纯化及降纤研究Cloning,expression,binantFibrinogenaseIVfromDeinagkistrodonacutusVenomMajor:PharmacologyName:XuLi—pengSupervisor:-meAbstractObjective:,iclocusonfibrinogen,smoking,operation,trauma,inflammation,infection,AMIetc,,,,itwasdifficulttoobtainpureproteinafterpurification,whichcausedtheclinicalpracticesideeffect,,andV中山大学博士学位论文重组五步蛇毒降纤酶的基因克隆、表达、纯化及降纤研究couldn’,PichiaPastoriswasamajorexpressionsystem,ithasmanyadvantagessuchasextracellularsecretionexpression,stableexpression,highproductionandthemulti—copiesofextragenecallrepeatedlyintegratingintothePichiaPastorisgenes,astheextraproteinsweresecretedintotheculturemedium,Ⅳicengineeringwasofgreatuseasforprovidingthisvaluableexperimentalfoundation,whichwouldbedeveloped【icengineeringdrugwithindependentintellectualproperityrights·Methodsandresults:ⅣgenefromDeinagkistrodonacutusvenomUsingantiseraagainstFibrinogenaseⅣtoscreeneDNAlibrary,ⅣDNAco
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