FQ―PCR检测BALF中结核杆菌―DNA对肺结核诊断的应用研究.docFQ-PCR检测BALF中结核杆菌一DNA对肺结核诊断的应用研究【摘耍】目的:对FQ-PCR检测BALF中结核杆菌-DNA对肺结核诊断的应用进行研究。方法:随机选収2013年12月-2014年11月期间本院收治的肺结核患者110例作为本次研究的对象,所有患者实施纤支镜检查、活检、刷检,BALF中结核杆菌-DNA行FQ-PCR检测,对检查结果进行分析研究。结果:在本次研究屮,%(44/110);FQ-PC检测的敏感性明显高于常规涂片镜检,差异有统计学意义(P〈0・05)o结论:在肺结核的诊断屮,FQ-PCR检测的敏感度高,操作简单方便,检测速度较快,是BALF中结核杆菌-DNA检查的重要方法,在肺结核的诊断中有重要的应用价值。【关键词】FQ-PCR检测;BALF;结核杆菌-DNA;肺结核诊断TheApplicationofMycobacteriumTuberculosis-DNAinBALFbyFQ-PCRfortheDiagnosisofPulmonary/TONGJian-lin,RAOChang-hong,HUANGBo.//MedicalInnovationofChina,2015,12(26):066-067[Abstract]Objective:Tostudytheapplicationofmycobacteriumtuberculosis~DNAinBALFbyFQ-PCRforthediagnosisofpulmonary・Method:110caseswithtuberculosisinourhospitalwererandomlyselectedfromDecember2013toNovember2014astheobjectsofthestudy,allpatientsweregiventhefiberopticbronchoscopy,biopsy,brushbiopsy,mycobacteriumtuberculosisDNAinbronchoalveolarlavagefluid(BALF)wasdetectedbyFQ-PCR,:Inthisstudy,44casesofpulmonarytuberculosis,%(44/110);thesensitivityofFQ-PCdetectionwassignificantlyhigherthanthatofconventiormlsmearmicroscopy,thediffereneewasstatisticallysignificant(P<)・Conelusion:Inthediagnosisofpulmonarytuberculosis,theFQ-PCRdetectionhashighsensitivity, simpleoperationandthedetectionspeedfaster,itisanimportantexaminationmethodofmycobacteriumtuberculosisDNAinBALFandhasimportantapplicationvalu
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