谷氨酸高产菌株的诱变选育研究.doc


谷氨酸高产菌株的诱变选育研究#
李友明1,刘峰2,胡建明1,吕阳爱1,郭继龙1,杨斌1,黄鹭强2,薛婷2,叶冰莹
2
,陈由强2,3**
5
10
(1. 福建省建阳武夷味精有限公司,建阳,354200;
2. 福建师范大学生命科学学院,福州,350117;
3. 福建发育与神经生物学重点实验室,福州,350117)
摘要:本文对多株产谷氨酸菌株进行紫外诱变、硫酸二乙酯诱变筛选研究,建立产谷氨酸菌
株的最佳诱变方法:紫外诱变时间 30s,致死率为 77%;硫酸二乙酯诱变时间 20min,致死
率为 79%。综合紫外与 DES 两种诱变方法对比来看,DES 诱变较紫外诱变更方便且效应也
较好,且筛选出产谷氨酸菌株 T3-1 的产酸含量高达 ,转化率为 %。
关键词:谷氨酸;紫外诱变;硫酸二乙酯诱变
中图分类号:TQ922
15
Study on Screening of High Yield Strain for Glutamic Acid
LI Youming1, LIU Feng2, HU Jianming1, LV Yangai1, GUO Jilong1, YANG Bin1,
HUANG luqiang2, XUE Ting2, YE Bingying2, CHEN Youqiang2,3
(1. Fujian Province Jianyang Wuyi MSG Co.,Ltd,Jianyang,354200;
20
2. College of life and Science,Fujian Normal University,Fuzhou,350117;
3. Fujian Key Laboratory of Developmental and Neural biology ,Fuzhou,350117)
Abstract:
In this paper ,glutamic acid producing strain were studied by UV irradiation,Diethyl
sulfate UV-DES mutation screening .The optimum mutation method for glutamic acid producting
strain was established: UV mutagenesis of 30s ,mortality 77% ;DES mutation of 20 min ,mortality
25
30
35
79% .Two kinds of uv and DES mutagenesis method by contrast, DES mutagenesis is more
convenient than ultraviolet mutagenesis, the mutant glutamic acid producing strain T3-1 yield up
to ,the conversion of %.
Key words: glutamic acid; UV irradiation; DES
0 引言
谷氨酸是一种酸性氨基酸