表达犬瘟热病毒H基因重组新城疫病毒的构建及其免疫原性研究.pdf.pdf

:~.—,苏华,(,黑龙江哈尔滨150001;,吉林长春130062;,黑龙江哈尔滨15OO30)摘要:为研制安全、有效的新型犬瘟热疫苗,本研究利用新城疫病毒(NDV)LaSota弱毒疫苗株反向遗传操作系统,构建出表达犬瘟热病毒(cDV)(H),并对其生物学特性进行鉴定,评估其作为犬瘟热活栽体疫苗的安全性和有效性。通过免疫荧光和westernblot试验证明了CDVH蛋白的正确表达;重组病毒株保持了LaSota亲本株的低致病性和高滴度鸡胚生长特性;重组病毒rLa-,可以诱导显著的CDV中和抗体反应。。关键词:犬瘟热病毒;血凝素蛋白;重组新城疫病毒中图分类号:$:A文章编号:(2012)—vectoredcaninedistempervirus(CDV)ineandinductionofCDVneutralizingantibodyTIANMei-jie,GEJin—ying,WANGXi-jun,FENGNa,MALiang3,SHUAILei,SUHua,GAOYu—wei,XIAXian—zhu,BUZhi—gao(,HarbinVeterinaryResearchInsitute,ChineseAcademyofAgriculturalSciences,Harbin150001,China;,AcademyofMilitarySciences,Changchun130062,China;,NortheastAgriculturalUniversity,Harbin150030,China)Abstract:inebasedonNewcastlediseasevires(NDV)inestrain,binantNDVwhichexpressedhemagglutinin(H)proteinofcaninedistempervirus(CDV,Rockborn一20/8strain),namedrLa-CDV—H,-CDV