水产品中己烷雌酚 己烯雌酚与双烯雌酚残留的毛细管电泳测定.doc

水产品中己烷雌酚_己烯雌酚与双烯雌酚残留的毛细管电泳测定.doc:..水产品中己烷雌酚、己烯雌酚与双烯雌酚残留的毛细管电泳测定林子俺,庞纪磊,黄慧,郑江南,张兰(福州大学化学化工学院食品安全分析与检测教育部重点实验室,福建福州350002)摘要:建立了毛细管电泳■紫外检测法测定水产品中己烷雌酚(HXS)、己烯雌酚(DES)及双烯雌酚(DIES)残留的新方法。研究了缓冲体系的酸度、浓度、添加剂、分离电压、进样时间及温度等对组分分离的影响。在检测波长为200nm,分离电压为20kV,运行缓冲液为50mmol/L硼砂-25mmol/LM氧化纳(,含30%的N,N二甲基甲酰胺)的条件下,3种目标组分在14min内达到基线分离。HXS、〜120、〜100、〜100mg/L范围内呈良好线性,相关系数(,)、、,〜,结果令人满意。关键词:毛细管电泳;水产品;己烷雌酚;己烯雌酚;双烯雌酚中图分类号:0657.&:A文章编号:1004・4957(2010)01・()055・()4doi:.1004-,DiethylstibestiolandDienestmlResiduesinAquaticProductbyCapillaryElectrophoresisLINZi-an,臥NGJiTei,HUANGHui,ZHENGJiang-nan,ZHANGLan(KeyLaborak^ryofAnalysisandDetectiontorFoodSafety,MinistryofEducation,DepartnentofChanistry,FuzhouUniversity,Fuzhou350002,China)Abstract:Afacilemethodwasdevelopedlorsmultaneousdeteiminationofhexestiol(HXS),dieth-ylstibestiol(DES)anddienestiol(DIES)residuesinaquaticproductbasedoncapillaryelectiophonesiscoupledwithultravioletdetectionTheinfluencesofsomeinportantfacerssuchasadditive,acidityandconcentrationofrunningbuffer,separationvoltage,injectiontimeandtemperatureweresystemicallyinvestigatedAtthedetectionwavelengthof200nm,agoodbaselineseparationofthreeanalyteswasobtainedwitliin14minbyusing50mmol/Lborax-25mmol/LNaOH()withadditiveof30%(byvolu