黄芪预处理对大鼠脑缺血再灌注损伤的保护作用 (作者:___________单位: ___________邮编: ___________) 【摘要】目的探讨黄芪(AS)对大鼠脑缺血再灌注损伤的预适应样保护作用。方法采用间断静脉推注AS模拟预适应的实验方法,观察大鼠双侧颈总动脉栓塞再灌血清中一氧化氮合酶(NOS)活性、及一氧化氮(NO)、丙二醛(MDA)含量以及脑组织Na+-K+-ATP酶、Ca2+-ATP酶活性的变化。结果AS(10,8 g/kg)预处理可明显降低血清中MDA含量,同时增加血清NOS、NO含量及脑组织Na+-K+-ATP酶、Ca2+-ATP酶活性和NO含量。结论黄芪预处理对大鼠脑缺血再灌注损伤具有缺血预适应样的保护作用。 【关键词】黄芪; 预处理; 脑缺血; 再灌注损伤; 保护 Abstract:ObjectiveTo study the protective effect of pharmacological preconditioning of astragalus (AS) against cerebral ischemic reperfusion injury in rats. MethodsCerebral ischemia reperfusion model was established by clamping mon carotid arteries. The activities of nitric oxide synthetase(NOS),Na+- k+ATPase,Ca2+ATPase and the contents of nitricoxide(NO) , malonaldehyde(MDA) were measured after pharmacological preconditioning of AS. ResultsThe pharmacological preconditioning of AS(10 g/kg、8g/kg) significantly reduced the contents of MDA and significantly increased the activities of NOS, Na+- k+ATPase,Ca2+ATPase and the contents of NO. ConclusionThe pharmacological preconditioning of AS has a protective action against cerebral ischemic reperfusion injury. Key words:Astragalus; Preconditioning; Brain ischemic; Reperfusion injury; Protection
