高效液相色谱法测定野生毛葡萄中的白藜芦醇.pdf.pdf

广西科学院学报 Journal ofGuangxi Academy ofSciences 2010。26(3):Z37~238,241 , August 2010 高效液相色谱法测定野生毛葡萄中的白藜芦醇* Determination ofResveratrol inWil dGrape by HPLC 周琳,陈秋虹 ZHOU Lin,CHEN Qiu—hong (广西分析测试研究中心,广西南宁530022) (Guangxi Research Center ofAnalysis and Testing,Nanning,Guangxi,530022,China) 摘要:为了建立整颗野生毛葡萄中白藜芦醇含量的高效液相色谱分析方法,采用组织分散匀质器将葡萄匀浆后用无水硫酸钠研磨干燥均匀样品,再用乙酸乙酯提取作为前处理方法,通过优化各种实验条件,选择色谱柱为SymmetryshielyTM RPl8柱( mmX 250mm,5^tm),%醋酸溶液(25:75),检测波长为306nm的实验方法进行实验。结果表明,~,%,%。该方法简单准确,重现性好,可以用于山葡萄酒原料野生毛葡萄的质量检测。关键词:高效液相色谱法白藜芦醇野生毛葡萄中图法分类号: 文献标识码:A 文章编号:1002—7378(2010)03—0237—02 Abstract:A method fordetermination ofresveratrol inthe whole wild grape by HPLC was grapes was homogenated byhomogeneous dispersion device and the anhydrous sodium sulfate was used togrind and dry thesamples were extracted byusing ethyl acetate as thepre-treatment optimizing avariety ofexperimental conditions,a SymmetryshielyTM RPl8( mm×250mm,5tlm)was selected as column,with acetonitrile: %HAC(25:75)as mobile Pbase and UV detection wavelength at results demonstrated the calibration curve ofresveratrol was linearbetween — and the average recovery was %with RSD equating to method was proved to be simple,rapid,accurate and can beused forthequality determination of