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吲哚丁酸和萘乙酸液相色谱检测.doc


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吲哚丁酸和萘乙酸液相色谱检测
高效液相色谱法同时测定蔬菜及水果中的吲哚丁酸和萘乙酸
王佳祥,王珊珊,赵平
(黑龙江省质量监督检测研究院,黑龙江哈尔滨,150050)
TM摘 要:采用高效液相色谱法,以Diamonsil C(250mm×,5μm)为色谱柱,甲醇:水:磷酸为18
60:40:(pH 3,4)作为流动相,检测波长为272nm的条件下测定萘乙酸和吲哚丁酸含量,试验结果表
明:在0,,相关系数r=, r=,最低检出浓度为吲萘
,、、%,%之间,相对
%,%之间,方法简便,准确。
关键词:吲哚丁酸;萘乙酸;液相色谱
High performance liquid chromatographic method for the simultaneous determination of
vegetables and fruits in the IBA and NAA
Wang Jia-xiang, Wang Shan-shan, Zhao Ping
( Heilongjiang provincial quality supervision and Inspection Institute, Heilongjiang Harbin, 150050)
Abstract: using high performance liquid chromatography, in order to DiamonsilTM C (250 mm x mm, 18
5 μ m) for chromatographic column, methanol: water: phosphate for 60:40:0. 35 (pH 3 ~ 4) as mobile phase and
detection wavelength of 272 nm conditions determination rootone and indolebutyric acid content, the test result indicated that: in 0 ~ mg/L scope inside sexual relationship is good, correlation coefficient r Yin = , r naphthalene = , the lowest detection concentration is mg/L, sample in the , , mg/L three to add at the level of the average is between % ~ %, relative deviation between % ~ %, the method is simple, accurate.
Key words: indolebutyric acid; Rootone, Li

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