心肌内注射epc.pdf

J. Cell. Mol. Med. Vol 13, No 4, 2009 pp. 664-679
Intracardiac injection of erythropoietin induces
stem cell recruitment and improves cardiac functions in a
rat myocardial infarction model
Christian Klopsch a, #, Dario Furlani a, #, Ralf Gäbel a, Wenzhong Li a, *, Erik Pittermann a,
Murat Ugurlucan a, Guenther Kundt b, Christiana Zingler c, Ulf Titze d, Weiwei Wang a,
Lee-Lee Ong a, Klaus Wagner f, Ren-Ke Li g, Nan Ma a, e, †, *, Gustav Steinhoff a, †
a Department of Cardiac Surgery, University of Rostock, Rostock, Germany
b Institute of Medical Informatics and Biometry, University of Rostock, Rostock, Germany
c Institute of Clinical Chemistry and Laboratory Medicine, University of Rostock, Rostock, Germany
d Institute of Pathology, University of Rostock, Rostock, Germany
e Zentrum für Biomaterialentwicklung, GKSS, Teltow, Germany
f Department of Anesthesia, Klinikum Südstadt, Rostock, Germany
g Division of Cardiac Surgery, Toronto General Hospital and the University of Toronto, Toronto, Canada
Received: July 12, 2008; Accepted: September 23, 2008
Abstract
Erythropoietin (EPO) protects the myocardium from ischaemic injury and promotes beneficial remodelling. We assessed the thera-
peutic efficacy of intracardiac EPO injection and EPO-mediated stem cell homing in a rat myocardial infarction (MI) model. Following
MI, EPO (3000 U/kg) or saline was delivered by intracardiac injection. Compared to myocardial infarction control group (MIC),
EPO significantly improved left ventricular function (n ϭ 11–14, P Ͻ ) and decreased right ventricular wall stress (n ϭ 8,
P Ͻ ) assessed by pressure-volume loops after 6 weeks. MI-EPO hearts exhibited smaller infarction size ( Ϯ % ve