激光粒度仪测定阿霉素纳米颗粒的粒径及粒径分布.docx

激光粒度仪测定阿霉素纳米颗粒的粒径及粒径分布.docx激光粒度仪测定阿霉素纳米颗粒的粒径及粒径分布
吕凤娇1陈晓耕2林华香1许小平1#
(1福州大学化学化工学院 福建福州350002; 2福建省省立医院 350000)
摘要：本文以阿霉素为模型药物， N-乙烯基吡咯烷酮为单体，通过反相乳液聚合法制
备了聚乙烯吡咯烷酮为载体的阿霉素抗癌纳米颗粒，并用激光粒度仪测量了纳米颗粒 的粒径大小和粒径分布，同时研究了乳化剂浓度、引发剂浓度、体系水量、反应温度 对纳米颗粒尺寸的影响。
关键词：阿霉素；纳米颗粒；激光粒度仪；粒径；粒径分布
中图分类号：TQ46 文献标识码：A
Determination of Doxorubicin-loaded nanoparticles size and
size distribution with laser particle size instrument
*1 Q A A JX
LV fen g-jiao CHEN xiao-ge ng LIN hua-xia ng XU xiao-p ing
(「School of Chemistry and Chemical Engineering, Fuzhou University, Fujian Fuzhou 350002;
2. Fujian Provincial Hospital Fuzhou 350000)
Abstract : Doxorubic in-loaded nan oparticles, using doxorubici n as model drug and polyv iny Ipyrrolido ne as matrix material, were prepared by in verse emulsi on polymerizati on in
this paper. The mean particle size and size distribution were measured by laser light scattering technique. The effect factors, such as the emulsifier, the initiator ，the water and the temperature， on the size and the size distribution of Doxorubicin-loaded nanopartic les were investigated. Keywords: doxorubic in; nan oparticle; laser light scatteri ng tech niq ue; size; size distributi on
纳米药物是国际上刚兴起的一种新型给药系统 ⑴，它以高分子材料为载体，药物通过
雾化法［2］、化学沉积法［3］、溶剂蒸发法⑷、薄膜-超声分散法［5］和乳液聚合法⑹等包覆在高分 子材料中。纳米药物制备的关键是控制粒子的大小和获得较窄且均匀的粒度分布，减少粒 子团聚现象，保证用药有效、安全和稳定。目前，测量纳米颗粒尺寸的仪器主要有透射电 镜、环境扫描电镜、 X射线衍射仪、激光粒度仪等。透射电镜和环境扫描电镜只能给出局
部粒径的大小；X射线衍射仪得到的只是单个晶包的尺