牛磺胆酸抑制肝癌细胞生长的体外研究.pdf

摘 要
胆汁淤积性肝病可能会转化为肝癌，其与各种胆汁酸紧密相关。但是，在胆汁淤积
性肝病转化为肝癌的过程中，目前胆汁酸对肝脏细胞和肝癌发展的作用机制尚不清楚。
牛磺胆酸（Taurocholic acid，TCA）在胆汁淤积时显著升高，可作为胆汁淤积性疾病诊
断的重要标志物。然而，在这一过程中 TCA 的含量显著升高的机理尚不清楚。此外，
目前 TCA 对肝癌细胞的作用主要集中在短期研究上、细胞活性无显著性变化，而且缺
乏对正常肝细胞的影响研究。因此，在本研究中，选取人正常肝细胞 THLE-2 作为实验
对照组模型，人肝癌细胞 HepG2 作为实验测试组模型，
（1）分别探究 TCA 短期干预细胞和体外模拟胆汁淤积性肝病下浓度的 TCA 长期
干预细胞后的细胞活性；
（2）分别测试 TCA 短期干预细胞和体外模拟胆汁淤积性肝病下浓度的 TCA 长期
干预细胞后的细胞凋亡情况和观察细胞凋亡状态；
（3）进一步从相关基因表达水平、细胞内活性氧和 Ca2+的产生上探究 TCA 短期干
预细胞和体外模拟胆汁淤积性肝病下浓度的 TCA 长期干预细胞的作用机制。

关键词: 牛磺胆酸；肝癌细胞；细胞抑制和凋亡的作用机制














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In Vitro Study of Taurocholic Acid Inhibiting Hepatoma Cell Growth

Abstract
Cholestatic liver disease, which may lead to liver cancer development, is closely
associated with a variety of bile acids. However, the mechanisms of some bile acid acting on
the liver during cholestasis and cholestatic liver diseases transforming into liver cancer remain
unclear. Taurocholic acid (TCA) is highly elevated in cholestatic liver cirrhosis patients.
Therefore, TCA can be used as an important marker for the diagnosis of cholestatic diseases.
However, the mechanisms of elevated TCA content involved in this process are unclear. In
addition, the effects of TCA on hepatoma cells are mainly focused on short-term studies, no
significant changes in cell viability, and lack of studies on the effects of normal
this study, the human normal hepatocyte THLE-2 was selected as the
experimental control group model, and human hepatoma cell HepG2 was used as the
experimental test group model.
(1) To investigate the cel