人胚胎干细胞定向分化为血管内皮细胞的初步研究_王彦.pdf

·66 · Journal of Tissue Engineering and Reconstructive Surgery, April 2010,
论著
· ·· ·
doi:.1673-
人胚胎干细胞定向分化为
血管内皮细胞的初步研究
王彦钱德俭尉真陆峻泓张文杰崔磊曹谊林
摘要目的诱导人胚胎干细胞定向分化为血管内皮细胞方法将来源于人囊胚内细胞团的细胞以含维甲
【】。 ES ,
酸的培养液经悬浮培养先形成拟胚体再将贴壁换含的培养液继续培养进行相差显微镜免疫荧光
, , EB , TGF-β1 , 、、
电镜等检测结果人胚胎干细胞经诱导后分化成为血管样结构Ⅷ因子免疫荧光检测呈阳性摄取实验
。, ,Dil-Ac-LDL
呈红色荧光透射电镜显示其与正常的人毛细血管非常相似结论该方法可诱导人胚胎干细胞分化为内皮细胞为
, 。,
骨创伤修复提供新的思路和手段
。
关键词骨创伤内皮细胞人胚胎干细胞诱导
【】
中图分类号文献标识码文章编号
【】 +1 【】 A 【】 1673-0364(2010)02-0066-04
Primary Study of Human Embryonic Stem Cell on Differentiation of Vascular Endothelial Cells WANG Yan1, QIAN
Dejian1, WEI Zhen2, LU Junhong3, ZHANG Wenjie3, CUI Lei3, CAO Yilin3. 1 Qianfoshan Hospital of Shandong Province,
Jinan 250014, China; 2 Shandong Province Hospital, Jinan 250021, China; 3 Department of Plastic and Reconstructive
Surgery. Shanghai Ninth People′s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China.
Corresponding author: CAO Yilin.
【Abstract】 Objective To investigate the feasibility of inducing human embryonic stem cell (ESC) into endothelial cells.
Methods Embryonic stem cell -derived human embryoid was cultured in suspension of retinoic acid (RA) and then
embryoid body (EB) formatted. EB was transferred to % gelatin coated dish and cultured on TGF-β1 culture medium by