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96T
猪圆环抗体〔PC-Ab〕试剂盒使用方法
检测范围:
1ng/L-45ng/L使用目de：
本试剂盒用于测定猪血清、血浆及相关液体样本中圆环抗体〔PHPantibodywhichwithHRPlabeledbecomeantibodytigen-enzyme-antibodycomplex,afterwashingCompletely,AddTMBsubstratesolution,,TMBsubstratebecomesbluecolorAtHRPenzyme-catalyzed,,accordingtothistojudgeHPexistinthesampleornot.
Materialsprovidedwiththekit
1
washsolution
20mlIbottle
7
StoppSolution
6ml1bottle
2
HRP-Conjugatereager
t6ml1bottle
8
Positivecontrol
3
Microelisastripplate
12well8strips
9
Negativecontrol
4
Samplediluent
6ml1bottle
10
Instruction
1
5
ChromogenSolutionA
6ml1bottle
11
Closureplatemembrane
2
6
ChromogenSolutionE
6ml1bottle
12
Sealedbags
1
Specimenrequirements
extractassoonaspossibleafterSpecimencollection,andaccordingtotherelevantliterature,,specimencanbekeptin-20topreserve,Avoidrepeatedfreeze-thawcycles.
Can'tdetectthesamplewhichconaN3,becauseNaN3inhibitsHRPactive.
AssayprocedureNumber:tosamplecorrespondmicrotitrationwellandNumberSequence,eachplateshouldbesetfemininecomparison2wells,masculinecomparison2wells,blankcomparison1well(don'taddsampHRPConjugatereagenttoblank
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