蜻蜓凤梨AfHSP基因的分离与功能鉴定 薛丙涛.pdf

: .
分子植物育种 32 ℃时乙烯的催花效率明显降低，推测
可能与相关热激蛋白有关。热激蛋白是生物体中普遍存在的一种调节蛋白，在植物体应对高
低温等逆境胁迫时发挥着非常重要的作用，基于蜻蜓凤梨的转录组测序数据和前期对蜻蜓凤
梨成花素基因 AfFT2(Aechemia fasciata FLOWERING LOCUS T 2)响应乙烯调控开花的研究，
筛选出 3 个可能与开花相关的热激蛋白基因并将其命名为 AfHsp20、、AfHsp70
(Heat shock protein 20, Heat shock protein , Heat shock protein 70)，分别对三个基因进行生
物信息学分析、基因功能鉴定及与 AfFT2 基因启动子的互作实验，初步证实 AfHsp20、
、AfHsp70 的表达量受温度影响且响应乙烯，其中 能与 AfFT2 启动子发
生互作。本研究为进一步研究高温条件下乙烯催花的作用机制提供参考，也为解决高温环境
中凤梨科植物催花问题提供相关理论依据。
关键词 蜻蜓凤梨; 乙烯催花; 热激蛋白; 酵母单杂交
Cloning and Analysis of AfHSP Gene from Aechemia
fasciataXue Bingtao1,2 Chen Ying1,2 Jing Yonglin2 Zhang Xuanbing1 Xu Li2 Wang Xiaobing2
Yan Hang2,3 Li Chunyan1,2 Li Zhiying2*
1 College of Horticulture, Hainan University, Haikou, 570228; 2 Institute of Tropical Crop Variety
Resources, Chinese Academy of Tropical Agricultural Sciences, Key Laboratory of Crop Gene
Resources and Germplasm Creation in South China (Ministry of Agriculture), Key Laboratory of
Tropical Crops Genetic Improvement and Innovation of Hainan Province, National Tropical
Medium-term Crop Bank, Danzhou, 571700; 3 College of Agriculture, Yangtze University,
Jingzhou, 434000
Abstract Aechemia fasciata is an important tropical flower. Due to the large uncertainties in its
flowering time and rate, ethylene is often used to promote flowers in production. However, the
flower urging efficiency of ethylene is significantly reduced when the temperature is higher than
32 ℃, HSP20, and HSP70 (Heat shock protein 20, Heat shock