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1 目录 1. 引言……………………………………………………………………………… 2 2. 适配体…………………………………………………………………………… 3 适配体的制备………………………………………………………………… 3 适配体的特点及优势…………………………………………………………… 3 适配体在分析领域的应用……………………………………………………… 4 3. 适配体传感器…………………………………………………………………… 4 光学传感器……………………………………………………………………… 4 电化学传感器…………………………………………………………………… 6 其他适配体传感器……………………………………………………………… 7 4. 总结……………………………………………………………………………… 8 参考文献……………………………………………………………………………… 8 附录………………………………………………………………………………… 10 致谢………………………………………………………………………………… 11 2 适配体传感器研究进展苏阳阳,安徽师范大学化学与材料科学学院摘要: 适配体是用指数富集配体系统进化( SELEX )方法,从随机单链寡聚核苷酸文库中筛选得到的一类能够与靶分子特定结合的单链寡核苷酸序列。不仅对靶分子有高特异性和高亲和力,而且具有自身稳定性好、无免疫原性、易体外合成、易功能化修饰与标记等特性。其在分析化学、环境监测、疾病治疗与新药研发等方面具有广阔的应用前景。本文简单阐述了适配体在多种传感技术方面的研究进展及应用,主要包括:光学传感器、电化学传感器、其他传感器等。并列举了近年来分析领域中的部分研究成果。关键词: 适配体;传感器;研究进展;应用 Advance in Aptamer-based Biosensors Su Yangyang, C ollege of Chemistry and Materials Science Abstract: Aptamers are the single – strand DNA or RNA oligonucleiotides selected by SELEX (systematic evolution of ligands by exponential enrichment) in vitro with high specificity and affinity for theit targets, and have been demonstrated to have advantages over antibodies with regard to chemical stability, readily availability, simple modifiability, and high flexibility in biosensor design for analyte detection. A ptamer have gained considerable interest in bio-analytical application in many fileds such as analytical chemistry, environmental supervision, disease treatment and drug development, etc. This article briefly discusses the research progress and application of aptamers ina variety of sensor technologies, including: optical sensors, electrochemical sensors, other sensors, etc. Following that, the multiplex analysis has been also stated in the development of aptamer-based biosensors and bioassay methods. Key words: aptamer; biosensor; research progress; application 1. 引言适配体是用指数富集配体系统进化( systematic evolution ofligands by 3 exponential enrichment )技术从随机的单链寡核苷酸文库中筛选得到的能够与靶分子进行特异性结合的单链寡核苷酸序列,可以是 DNA 或