还原性谷胱甘肽对急性坏死性胰腺炎肺损伤的影响.doc

1 还原性谷胱甘肽对急性坏死性胰腺炎肺损伤的影响【关键词】胰腺炎, 急性坏死性;肺损伤;细胞因子;谷胱甘肽; 治疗 Effect of reduced glutathione on lung injury in rats with acute necrotizing pancreatitis 【 Abstract 】 AIM: To investigate the changes of transcription factor nuclear factor κB (NF κ B), TNF α, IL1 β, IL18, myeloperoxidase (MPO) in the lung of rats with acute necrotizing pancreatitis (ANP) and explore the effects of reduced glutathione (GSH) on lung injury. METHODS: Wistar rats (n=72) were divided into 3 groups at random, ANP group, GSH treated group, and sham operation (SO) group. Animals in the 3 groups were killed 6, 12 and 18h after operations and lung tissue, pancreas tissue were harvested. The expressions of IL18, IL1 β, TNF α in lung tissue were measured by immunohistochemistry. MPO was determined by chromatometry. Intrapulmonary expression of NFkBp65 mRNA was detected by 2 RTPCR. The histopathology of lung and pancreas was observed under the light microscope. RESULTS: The expressions of NF κB mRNA, IL18, IL1 β, TNF α and the activity of MPO in lung tissue were much higher at 6, 12, 18h in ANP group, with statistical pared with the SO group () and the levels of NF κB mRNA, IL1 β and TNF α reached the peak at 12 h. After treatment with GSH, the levels of IL18, IL1 β, TNF α became lower at 6, 12, 18h and NF κB mRNA, MPO were decreased at 12h ( vs ANP group). CONCLUSION: All of NF κB, TNF α, IL1 β, IL18 and MPO have something to do with acute lung injury in experimental acute necrosis pancreatitis. GSH may inhibit the activity of NF κB and neutrophil in lung tissue, decrease the concentrations of inflammatory cytokines (TNF α, IL1 β, IL18), thus exerting the preventive effect against lung injury. 【 Keywords 】 pancreatitis, acute necrotizing; lung injury; cytokine; glutathione; therapy 【摘要】目的:观察急性坏死性胰腺炎(ANP) 大鼠肺组织核因子κ B(NF κ B)mRNA , 细胞因子( TNF α, IL1 β, IL18 ), 髓过氧化物酶( MPO ) 的变化, 探讨还原性谷胱甘肽( GSH ) 3 治疗肺损伤的机制. 方法:72只 Wista r 大鼠随机分成3组: ANP 组, GSH 组, SO 组(假手术组) . 于术后 6,12,18 h取肺组织供实验用. 免疫组化测定肺中 IL1 β,