反相高效液相法测定温胃舒片中补骨脂素和异补骨脂素的含量.doc

反相高效液相法测定温胃舒片中补骨脂素和异补骨脂素的含量.doc反相高效液相法测定温胃舒片中补骨脂素和异补骨脂素的含量
作者:蔡皓, 张科卫, 李伟, 崔小兵
【摘要】目的建立一种测定温胃舒片中补骨脂素和异补骨脂素含量的反相高效液相法。方法采用Kromasil C18柱,以甲醇水(55∶45)为流动相,紫外检测波长246 nm,流速1 ml·min-1。结果在所使用的色谱条件下,补骨脂素和异补骨脂素出峰位置未见干扰,~ μg·ml-~ μg·ml-1浓度范围内线性关系良好(r= 9)。%%,% (n=6)% (n=6)。结论该法可信度高,操作简便,重现性好,可用于温胃舒片生产上的质量控制。
【关键词】温胃舒片; 补骨脂素; 异补骨脂素; 反相高效液相法; 定量分析
Determination of the Contents of Psoralen and Isopsoralen Contained in acrocephalae, Fructus Crataegi, Fructus Mume, Fructus Amomi, Pericarpium Citri Reticulatae and Fructus Psoraleae by the method of boiling ing and nourishing stomach,promoting "qi" circulation to relieve pain. It can mainly be used to treat patients and symptoms for chronic atrophic gastritis and gastric abscess, abdominal distension, belching, anorexia, chilly, acratia etc. caused by chronic gastritis in clinical application. Psoralen and Isopsoralen are the major active constituents of Fructus Psoraleae and also the main effective contents in orphological and MTT assays in vitro[2]. The chemical structures of Psoralen and Isopsoralen are shoination of the contents of Psoralen and Isopsoralen, the major analytical methods reported in the literatures are UV-spectrophotometry, TLCScanning, GC, CGC, SFECGC and HPLC[3~8]. Since HPLC has the advantage of convenient and good repetition, thus in the study of quality c