压电免疫传感器阵列检测尖吻蝮蛇毒的初步研究.doc

压电免疫传感器阵列检测尖吻蝮蛇毒的初步研究.doc压电免疫传感器阵列检测尖吻蝮蛇毒的初步研究
刘明华1,姚婕2,向强1,张波2,府伟灵2 (第三军医大学附属西南医院:1急救部;2检验科。重庆市,400038)
摘要: 目的探讨以压电传感阵列技术检测尖吻蝮蛇毒的可行性; 方法构建2×5 的AT切向、基频10 MHz 的石英晶体微阵列,将尖吻蝮蛇毒抗体巯基化,通过自组装技术固定在石英晶体金膜电极表面,检测不同浓度的尖吻蝮蛇毒标准液。结果尖吻蝮蛇毒压电免疫传感器阵列上,最适抗体固定浓度为3 ug/ml,尖吻蝮蛇毒与相应抗体反应时间为40分钟, ug/ml,~ ug/ml范围时,与晶体频率变化之间呈良好的线性关系。结论采用压电免疫传感器阵列检测尖吻蝮蛇毒响应特性良好,具有灵敏度高、操作简单、不需标记等优点,是实现快速仪器化检测蛇伤的可能途径。
关键词:压电;免疫传感器;阵列;蛇毒;尖吻蝮毒蛇
中图法分类号: 文献标识码:A
Study on piezoelectric quartz crystal microarray immunosensor of detecting Agkistrodon acutus
LIU Ming-hua1, YAO Jie2, XIANG Qiang1, ZHANG Bo2, FU Wei-ling (1 Department of Emergency,2 Department of of Laboratory Medicine, Southwest Hospital, Third Military Medical University ,Chongqing 400038 ,China)
Abstract: Objective To access the possibility of detecting Agkistrodon acutus by the piezoelectric microarray immunosensor. Method Building the microarray immunosensor with quartz crystal of 10 MHz AT-cut and gold-coated electrodes in 2×5 style. Immobilizing of venom antibody was plished by Thiol method and self–assembling, then detecting venom in serials concentration. Results Experimental results showed that the most suitable concentration of antibody was ug/ml, the response time was 40 minutes,the piezoelectric immunosensor had good response to homologous venoms, the frequency shifts were linearly dependent on the venom concentration in the range of 0.