SOD酶的资料2.doc

SOD酶的资料2
SOD
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Drought brings about different biochemical responses in plants in order to minimize its deleterious effects. Drought induces an oxidative stress in Lotus
corniculatus leaves, measured as an increment in lipid membrane peroxidation and in situ detected superoxide. As a result, total superoxide dismutase (SOD: .
) activity increases after a 4-h drought, when the hydric potential has decreased to ? MPa. Assays with specific inhibitors suggest the presence of MnSOD, Cu/ZnSOD and FeSOD activity in L.
corniculatus plants, contribute 60, 30 and 10% of total SOD activity, respectively. The Cu/ZnSOD isoform proved to be the most responsive to drought showing a remarkable increase in its activity as that corresponds to the induction of three different isoenzymes.
Expression analysis of SOD isoforms revealed an increase in Cu/ZnSOD transcripts with a maximum
accumulation 4 h after drought imposition. The possible role of SOD enzymes as an antioxidant protector
system under water stress conditions in
L. corniculatus is discussed.
超氢化物歧化酶的研究与应用,化学与生物
2004,
3:
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Relative water content (RWC) was determined as
described earlier [20]. Plant hydric potential (_) was
measured using a pressure chamber [21]. Hydric potential, RWC, analytical and biochemical determinations were done in pooled samples of leaves harvested after the stated time span.
[20] M. Antol?′n, J. Yoller, M. Sa′nchez-D?′az, Effects of temporary drought on nitrate fed and nitrogen fixing alfalfa plants, Plant Sci. 107 (1995) 159–165.
――Omar Borsani a,1, Pedro D?′az a,b,1, Mar?′
superoxide dismutase in Lotus corniculatus Plant Science 161 (2001) 757–Drought, created by withholding water, led to significant reductions in the absolute (?%) and relative (?
%) water content of chives (Allium schoenoprasum) leaves, a significant rise in the osmolarity of the leaf sap
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(+%) and a loss of leaf transpiration (leaf diffusion res