高效液相色谱法测定食醋和酱油中苯甲酸钠和山梨酸钾含量.doc

高效液相色谱法测定食醋和酱油中苯甲酸钠和山梨酸钾含量
高效液相色谱法测定食醋和酱油中苯甲酸钠和山梨
酸钾含量
HPLC in soy sauce and vingar sodium
benzoate potassium sorber content
学生姓名:XXXX 学号:XXXX 班级:XXXX 指导老师:XXXXXX
Abstract: [purpose] with high-performance liquid chromatography (HPLC) in soy sauce and vinegar and sodium benzoate sorbic acid potassium content.【 methods 】 the RP-HPLC method with Hyperclone BDS using C18 column (150 x nm, 5 um, phenomenex) for chromatographic column; Mobile phase: methanol: mol/l ethanol amine (20:80); The column
temperature 25 ℃, velocity mol/min, detected wavelength 230 nm, into the sample weight (standard sample quantity: , 5, 10, 15, ; the samples into the sample weight ul: 5 ul).
【 results 】: Sodium benzoate in soy sauce with the concentration of was ; potassium
sorbate; sorbic acid potassium, no sodium benzoate in vinegar 结论:高效液相色谱法具有高速、高效、高灵敏度的优点,所以数据准确,用外标法计算结果操作简单,计算方便,得出样品中苯甲酸钠的含量。
Conclusion: high performance liquid chromatography with speed, high
efficiency, high sensitivity of advantage, so exact data, with external standard method calculation results simple operation, easy calculation, concluded that the content of sodium benzoate samples.
关键词:高效液相色谱法外标法苯甲酸钠山梨酸钾 Keywords: high performance liquid chromatography external standard method sodium benzoate potassium sorbet
前言:苯甲酸钠,化学式:C6H5O2Na,是苯甲酸的钠盐,苯甲酸钠是很常用的防腐剂,有防止变质,延长