白花蛇舌草中总三萜酸的提取纯化工艺.doc

白花蛇舌草中总三萜酸的提取纯化工艺
蒙瑞波1,汤庆发1,曾永长2,郭阳1,傅钧庭1,范昊宁1,罗佳波*
(1. 南方医科大学中药新药实验室广东省中药制剂重点实验室,广州 510515;,广州 510633)
[摘要] 目的: :以总三萜酸提取率为指标,利用正交实验法考察最佳提取工艺;以静态吸附和解析实验筛选树脂型号,:最佳提取工艺为: 70%乙醇10倍量回[第一作者] 蒙瑞波,中药师,南方医科大学在读硕士研究生,主要从事中药新制剂开发研究,联系电话:**********,邮箱:mengruibo2005@
[通讯作者] 罗佳波,教授,博士生导师,国家杰出贡献中青年专家,主要从事中药复方与新制剂开发研究。联系电话:020-61648266,邮箱:ljb@
h。AB-8型大孔树脂最佳纯化工艺为上样流速2 BV·h-1,上样液pH值5, mg·mL-1,洗脱剂pH值7,洗脱乙醇浓度80%,洗脱速度2BV·h-1。结论:该工艺操作简单、稳定可行,重复性好,适合白花蛇舌草总三萜酸的提取纯化。
[关键词] 白花蛇舌草;三萜酸;正交设计;提取纯化
The extraction and purification process of total triterpene acids in Hedyotis Diffusa Willd
MENG Rui-bo1,TANG Qing-fa1,ZENG Yong-chang2 ,GUO Yang1,FU Jun-ting1, FAN Hao-ning1,LUO Jia-bo*
(1. Southen university key Lab of TCM and new drug ;Guangdong provincial key Lab of TCM, Guangzhou 510515;2. Yangtze River Pharmaceutical Group Guangzhou Harris pharmaceutical ,Guangzhou 510633)
[Abstract] Objective: To optimize of extraction and purification process of triterpene acids in Hedyotis Diffusa : To investigate the optimum extraction process by Triterpenoid acid extraction rate ; Screening resin model by static adsorption and parsing experiments,To investigate the optimal elution conditions by dynamic adsorption and resolution : The optimum extraction process was reflux extraction with 10- fold 7