过氧亚硝基对蛋白质的硝化..ppt

主要研究方向:
硒的生物无机化学
硝化对蛋白质结构、功能的影响
胰岛素信号传导体系~糖尿病
多肽蛋白类药物的非注射给药途径的研究(纳米药物)
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Purification of the Newly Found Selenium-Containing Proteins in the Arterial Wall and Brain of the Rat, Biochem Biophys mun. 2000, 270(3): 688-694.
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过氧亚硝基对蛋白质的硝化
及其生物学意义
黄开勋,池泉,何兴牧,徐辉碧
华中科技大学化学系,武汉430074
(国家自然科学基金资助)
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Giuseppe L et al. Free Radic Biol & Med. 1998,25: 392–403
The rate constant of the reaction of O2•‾ with •NO is larger (~1010 M–1•S–1) than with SOD (1–2×109 M–1•s–1), and therefore •NO sometimes petes SOD for O2•‾. The reaction of •NO with O2•‾ leads to the diffusion-controlled formation of peroxynitrite anion (ONOO‾)
一、研究背景
过氧亚硝基的产生与蛋白质硝化
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Peroxynitrite reaction pathways
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The free radical mechanism of tyrosine nitration
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蛋白质硝化的选择性
蛋白质印迹(抗NT)观察到的硝化蛋白与考马斯亮蓝染
色的凝胶上的主要条带并不总是吻合,这说明蛋白质
的硝化不仅仅由蛋白质的大小和含量所决定。
蛋白质结构上的差异决定了硝化是一个选择性修饰特
定Tyr残基的过程。

表面暴露
靠近带负电荷残基
金属活性中心的催化
FIGURE
Location of surface-exposed tyrosines in glutamine synthetase.
Barba