桑椹抗氧化活性的初步研究.doc

桑椹抗氧化活性的初步研究
【摘要】目的:桑椹是既是食用又是药用的水果之一,具有很高的营养价值,本文对它所含的抗氧化活性成分进行了研究。方法:新鲜桑椹的甲醇提取物用乙酸乙酯萃取后,通过硅胶柱层析、薄层层析等进行分离,得到的馏分用DPPH法测定其抗氧化活性。结果:经过两次分离,各得到12个和7个馏分,大部分的馏分显示出了很强的抗氧化活性。结论:日常食用的药食两用水果桑椹中含有抗氧化活性成分,可以通过日常饮食的摄入达到抗氧化的目的。
【关键词】桑椹;抗氧化活性;DPPH法
【Abstract】 Objective Mulberry is one of both edible and medicinal fruits with high nutrition. The ponents were isolated and analyzed. Method The fresh mulberry were extracted with methanol, and after concentration under reduced pressure, the aqueous residue was partitioned with EtOAc to give EtOAc extract. After isolated and purified by a silica gel column and thin layer chromatography, gained fractions were supplied to measure the antioxidative activity by DPPH method. Result 12 fractions and 7 fractions were gained after isolated twice. Most of the fractions showed the very strong antioxidative activity. Conclusion There are ponents in mulberry, and antioxidation can be achieved by intake of mulberry.
【Key words】 mulberry; antioxidative activity; DPPH method
桑椹,又名桑果、桑子,味甘、性寒,具有生津止渴、滋阴补血、补肝益肾、固精安胎、乌须黑发、聪耳明目、安神养心、润肠通便、健步履、利关节、去风湿、解酒等功效,常用于医治糖尿病、肝肾阴虚、失眠耳鸣、津液不足、风湿、便秘、须发早白、神经衰弱[1]等。现代医学研究发现,桑椹还具有防癌抗诱变、增强免疫力、驻颜抗衰老、促进造血细胞生长、降低血糖血脂[2]等方面的保健功能。本研究旨在通过提取桑椹鲜果中的抗氧化活性物质,利用DPPH法测定各馏分的抗氧化活性。
1 材料与方法
材料。作为实验材料的桑椹为济南近郊所产的水果,从济南市果品批发市场购得。
仪器。 SB-1000型旋转蒸发仪:上海爱明仪器有限公司;UV754紫外可见分光光度计、FA2004B型电子天平:上海精密科学仪器有限公司;SHB—Ⅲ循环水式多用真空水泵:郑州长城科工贸有限公司;ZF7C