樟子松松针石蜡切片制备（英文版）.pdf

樟子松松针石蜡切片制备林清鹏,张林波,王健*(吉林农业大学生命科学学院,吉林长春 130118)摘要[目的]以樟子松松针为材料,探索一套适合樟子松松针制备石蜡切片的条件。[方法]在传统植物石蜡切片技术的基础上,结合松针的结构特点,对固定、脱水、粘片、染色等环节进行了系列摸索。[结果]FAA 固定剂配比中采用 70%乙醇;粘片时材料置于 40 ℃水浴锅中展开,用防脱载玻片捞取,置 35 ℃烘箱24 h; 松针切 8 滋m 厚度, 染色条件为伊红 5min,苏木精 1 min。[结论]获得的条件可强化固定和脱水效果,粘片操作简便,不易脱片,染色效果较好。关键词樟子松;松针;石蜡切片基金项目吉林农业大学 2012 年国家级大学生创新创业训练计划项目(2012093025)。作者简介林清鹏(1991- ),男,福建宁德人,专业:制药工程。* 通讯作者,E鄄mail:Zhenkong鄄wj999@。收稿日期 2013鄄09鄄24修回日期 2013鄄10鄄26Preparation of Paraffin Section of Pinus sylvestrisNeedlesQingpeng LIN, Linbo ZHANG, Jian WANG鄢College of Life Sciences, Jilin Agricultural University, Changchun 130118, ChinaSupported by the 2012 Innovation and Business Start鄄up Training Program for theNational College Students of Jilin Agricultural University(2012093025).鄢Corresponding author. E鄄mail: ******@Received: September 24, 2013 Accepted: October 26, 2013AAgricultural Science & Technology, 2013, 14(11): 1575-1577, 1582Copyright 訫 2013, Information Institute of HAAS. All rights reserved Agricultural Basic Science and TechnologyAbstract [Objective] Taking Pinus sylvestris needles as materials, a set of test con鄄ditions suitable for the preparation of paraffin sections of Pinus sylvestris needleswas determined. [Method] Based on the traditional method for paraffin sectionspreparation, steps including fixation, dehydration, adhering to slides and stainingwere investigated taking the structural