HPLC法测定大黄碳酸氢钠片中的大黄素和大黄酚的含量.doc

HPLC法测定大黄碳酸氢钠片中的大黄素和大黄酚的含量
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【摘要】[目的]建立高效液相色谱法测定大黄碳酸氢钠片中大黄素和大黄酚的含量。[方法] 采用高效液相色谱法;色谱柱为Shimpack clcODS(150×,),流动相为甲醇%磷酸(85∶15),,检测波长为254nm。[结果] %、%,RSD=%、%(n=9)。[结论]本法简便、准确,重现性良好,可用于大黄碳酸氢钠片的质量控制。
【关键词】高效液相色谱法; 大黄碳酸氢钠片;大黄素;大黄酚

Abstract:[Objective] TO establish an HPLC method for the determination of emodin and chrysophanol in rhubarb sodium bicarbonate tablets.[Methods] An HPLC method was applied; Chromatographic column was Shimpack clcODS(150×,);The mobile phase was methanol% phosphoric acid (85:15);Velocity of flow was ;The detaction wavelength was 254nm.[Resulet] The linearty of this method
was and chrysophanol average recovery rates were %,%;RSD=%,%(n=9).[Conclusion] This method is simple,accurate with strong reproducibility,and can be used for the quality control of rhubarb sodium bicarbonate tablets.
Key words:HPLC; rhubarb sodium bicarbonate table; emodin; chrysophanol
大黄碳酸氢钠片是复方制剂,主要成份是由大黄、碳酸氢钠、薄荷油等组成,是助消化药及抗酸药类非处方药药品,主要用于食欲不振、胃酸过多[1]。大黄碳酸氢钠片的质量标准收载于卫生部药品标准化学药品及制剂第一册中[1],并没有对大黄进行含量测定的内容,本文采用高效液相色谱法对其大黄素与大黄酚的含量进行测定。
1 仪器、样品及试药
Agilent 1200高效液相色谱仪,Agilent Technologies 1200 DAD检测器,Agilent chemststion工作站。大黄素对照品(中国药品生物制品检定所,批号:110756200110),大黄酚对照品(中国药品生物制品检定所,批号:110796200513),大黄碳酸氢钠样品(市售,上海黄海制