HPLC法测定祛痹舒肩丸中阿魏酸的含量.doc

HPLC法测定祛痹舒肩丸中阿魏酸的含量
(作者:___________单位: ___________邮编: ___________)
作者:申强陈燕忠吕竹芬庄义修
【摘要】目的:建立以高效液相色谱法测定祛痹舒肩丸中阿魏酸含量的方法。方法:色谱柱为C18柱( mm×250 mm,5 μm),流动相为乙睛-%磷酸溶液(17∶83),检测波长为323 nm, mL/min,柱温为室温。结果:阿魏酸在(~)mg/mL范围内浓度与峰面积线性关系良好(r=,n=7),%,%(n=6)。结论:本方法简便、灵敏、准确,可用于祛痹舒肩丸的质量控制。
【关键词】高效液相色谱法祛痹舒肩丸阿魏酸
Abstract Objective:To develop a HPLC method for determination of ferulic acid in Qubi Shujian :The analysis was carried out on a Diamonsil C18 column ( mm×250 mm,5 μm) with a mobile phase acetonitrile-% phosphoric acid (17∶83). The flow rate was mL/min at the room temperature and the UV detection wavelength was 323 :The calibration curve was linear at the ra
nge of(~)mg/mL for the ferulic acid (r=,n=7).The average recovery rate was % and RSD was % (n=6).Conclusion: The method was rapid, sensitive and accurate, and could be used for the quality control of this preparation.
Key words HPLC;Qubi Shujian Pills;Ferulic acid
祛痹舒肩丸是由桂枝、羌活、黄芪、三七、当归等十四味中药组成的中药复方制剂,临床主要用于治疗肩关节性周围炎,以治疗风寒痹为主[1]。本品原质量标准中无阿魏酸的含量测定方法,不利于制剂的质量控制。本文参照《中国药典》[2],建立了HPLC法测定祛痹舒肩丸中的阿魏酸的含量。本法操作简便,灵敏度高,重现性好,结果准确可靠,可作为该药的质量控制标准。
1 仪器与试药
Dionex P680高效液相色谱仪,Dionex UVD170U可变波长检测器,Chromeleon色谱工作站,Sartorius BS224S电子分析天平,UV-1101紫外可见分光光度计,KQ-300VDE型双频数控超声波清洗器。阿魏酸对照品(中国药品生物制品检定所,批号:110773-200611,供含量测定用),祛痹舒肩丸(汕