ICAM1在实验性自身免疫性脑脊髓炎发病机制中的作用.doc

ICAM1在实验性自身免疫性脑脊髓炎发病机制中的作用
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作者:赵亚朴王红刘慧敏王秀卿黄庆军
【摘要】目的检测实验性自身免疫性脑脊髓炎(EAE)大鼠血脑屏障中血管内皮细胞细胞间粘附分子1(ICAM1)的表达,并分析其表达与EAE发病的关系。方法取EAE组和对照组大鼠的脑组织,石蜡切片,免疫组化染色,图像扫描系统观测,图像分析软件进行灰度分析。结果 EAE组大鼠脑血管内皮细胞上ICAM1的表达明显深于对照组。±,±,两组差异非常显著()。结论大鼠EAE时脑血管内皮细胞ICAM1的表达上调,提示ICAM1是与EAE发生相关的重要粘附分子。
【关键词】免疫组织化学法; ICAM1; 血脑屏障; 实验性自身免疫性脑脊髓炎
【Abstract】 Objective To investigate the relationship between the expression of ICAM1 in the bloodbrain barrier and the experimental autoimmune encephalomyelitis(EAE). Methods Brain of EAE as well as control animals were taken and embedded in the sections were stained by immunohistochemical method. Then the sections were o
bserved by Nikon image scanning system and measured the gray value by IPP image analysis software. Results The expression of ICAM1 in the epithelial cell of endangium of EAE rat’s brain was higher than that in control. Conclusion The increase of the expression of ICAM1 in the epithelial cell of endangium of EAE rat’s brain indicated that ICAM1 was a necessary adhesion molecule in the developmental process of EAE.
【Key words】 Immunohistochemical method;Intercellular adhesion molecular1;Bloodbrain barrier;Experimental autoimmune encephalomyelitis
多发性硬化症(multiple sclerosis,MS)可能是一种与遗传、病毒感染、地理环境等诸多因素有关的自身免疫性疾病[1]。实验性自身免疫性脑脊髓炎(experimental