TSA对甲状腺鳞癌细胞株SW579增殖的影响 (作者:___________单位: ___________邮编: ___________) 【摘要】目的探讨曲古抑菌素A(trichostatin A,TSA)对甲状腺鳞癌细胞株SW579生长增殖的影响。方法分别以终浓度为25、50、100、200、400 nmol/L的TSA作用于SW579细胞株,在倒置显微镜下观察细胞生长情况及形态变化;采用四甲基偶氮唑蓝比色法(MTT)检测细胞的体外增殖、吖啶橙染色荧光显微镜观察细胞形态及细胞凋亡、流式细胞术检测细胞凋亡率的改变。结果 TSA作用后对细胞的增殖有抑制作用;吖啶橙染色后光镜下观察发现有典型的凋亡小体生成;流式细胞仪分析凋亡率随TSA浓度的升高而升高(P<)。结论不同浓度的TSA可抑制SW579细胞增殖,促进细胞凋亡,且呈剂量依赖性。 【关键词】曲古抑菌素A 甲状腺鳞癌细胞增殖细胞凋亡 Abstract:Objective To study the effects of trichostatin A (TSA) on apoptosis and proliferation of thyroid squamous cell carcinoma SW579. Methods Treated with different doses of TSA (final concentration of 25 nmol/L,50 nmol/L, 100 nmol/L, 200 nmol/L, 400nmol/L), the cells were observed with an inverted microscope;The proliferation activities of cell line were determined by using methyl thiazolyl tetrazolium(MTT) assay. Cell morphologies and apoptotic were observed by fluorescence microscope stained with acridine orange . Flow cytometry was adopted to examine apoptotic rate. Results TSA inhibited significantly the growth of cell line and the effect was with a dose dependent manner. Typical apoptotic morphologic feature was discovered under fluorescence microscope. The apoptotic peak was detected with FCM and apoptotic rate increased with the concentration raised. Conclusions TSA can inhibit the proliferation and induce apoptosis in the thyroid cancer cells with a dose-dependent m
