急性放射性分泌性中耳炎新西兰兔模型的建立及研究.doc

急性放射性分泌性中耳炎新西兰兔模型的建立及研究
作者:张志钢陈洽鑫毛永林郑亿庆刘翔
【摘要】【目的】建立急性放射性分泌性中耳炎的新西兰兔动物模型,并观察不同辐射剂量、方式对中耳的影响。【方法】 72只健康的新西兰兔随机分成4组(每组18只),包括对照组(A组)和3个实验组B、C、D。 B组施加辐射剂量为30 Gy/次× 1次,C组为10 Gy/次× 3次,D组为10 Gy/次× 6次。在照射前及照射后第1天,第1、2、4、8、12周分别行耳内镜、中耳CT、声阻抗检查,并在放疗后的每个观察时间点处死各个组的3只兔子,取其鼻咽黏膜、听泡行组织学检查。【结果】初步建立了急性放射性分泌性中耳炎的新西兰兔动物模型。【结论】利用新西兰兔建立放射性分泌性中耳炎的动物模型是可行的,但单次大剂量的电离辐射是成功建模的关键。
【关键词】辐射; 分泌性中耳炎; 动物模型
Abstract: 【Objective】 To establish a model of radiological secretory otitis media using New Zealand rabbits, and to observe the results of different radiation dosages and modalities. 【Methods】 Seventy-two healthy New Zealand rabbits were randomly divided into four groups (18 for each), including one control group named group A, and three experimental groups named group B, C and D. Group B was administered with a radiation dose of 30 Gy per for once, while group C with 10 Gy per for three times and group D with 10 Gy per for six times. Before given the radiation and on the 1st day, 1st week, 2nd w
eek, 4th week, 8th week and 12th week after, endotoscopy was carried out respectively as well as CT, auditory impedance test. Meanwhile, 3 rabbits each group for each time were sacrificed and specimens of bullae and mucous membrane of nasopharynx were collected for histological examination. 【Results】 Preliminary ess was gained in the establishment of a animal model of secretory otitis media using New Zea