电针对大鼠胃粘膜胃泌素和EGFR表达及AgNOR的影响.doc

电针对大鼠胃粘膜胃泌素和EGFR表达及AgNOR的影响
作者:赵保民,黄裕新,张伟,赵宁侠,刘杰,王庆莉,王连刚
【关键词】电针
关键词: 电针;足三里;胃泌素;受体,表皮生长因子;胃粘膜;免疫组织化学

摘要:目的观察电针足三里穴对胃粘膜细胞受体表达及核增殖的影响,探讨针灸作用机制. 方法 75只SD大鼠随机分段分为5组,在清醒状态下针刺实验,SABC法免疫组化染色观察胃粘膜细胞胃泌素(Gas)、表皮生长因子受体(EGFR)表达程度,AgNOR特染观察胃腺细胞增殖情况. %,%;电针三阴交对EGFR表达无影响,%;联合穴位组Gas,%,AgNOR特染各组核内嗜银颗粒无明显差异. 结论电针足三里可提高胃粘膜细胞Gas和EGFR表达率,改变细胞分子结构,.

Keywords:electroacupuncture;zusanli;gastrin;epidermal growth factor receptor;gastric mucosa;im-munocytochemistry

Abstract:AIM To observe the effect of electroacupuncture stimulation of zusanli on the expression of Gas and EGFR in gastric mucosa in order to probe mechanisms of 75SD rats were randomized into5groups:Control group,non-point group,zusanli group,sanyinjiao group and unite point rats were experimented on staining by means of SABC or SP technique was used to observe the expression,location and distribution of Gas and EGFR in gastric ,gastric mucosa cells were specially stained with EGFR and Gas in gastric mucous tissue of the zusanli group after EA showed a positive expression significantly higher than those of the control and the non-acu-point expressions of Gas and EGFR were en-hanced significantly(%%,respectively)in zu-sanli expression of Gas in gastric mucous tissue were enhanced significantly(%),but the expression of EGFR kept stable in Sanyinjiao expression of Gas was enhanced as significantly as that of EGFR in zu-san ,nuclear argentaffin particles that were spe-cially stained with AgNOR didn’t change Electroacupuncture stimulation of zusanli can enhance the expression rate of EGFR and Gas in gastric mucoas,but exert no effects on the proliferation of gastric mucous cells.

0 引言

中医认为,经脉与脏腑相关,针刺经络腧穴可调节脏腑功能[1] .但针刺如何调节脏腑功能,经穴与脏腑功能联系的本质是什么?尚不清楚.