虎杖不同酒制品大黄素及大黄酸含量比较.doc

虎杖不同酒制品大黄素及大黄酸含量比较

【摘要】目的探讨酒制对虎杖中大黄素和大黄酸的影响。方法采用高效液相色谱法对其不同酒制品进行含量测定。结果不同酒制品中大黄素含量由高到低的顺序为:酒润品>酒煮品>酒炒品,大黄酸含量则相近。结论不同酒制方法及炮制温度对大黄素含量有一定影响。
【关键词】虎杖炮制大黄素大黄酸高效液相色谱
Abstract:ObjectiveTo investigate the effects of the different wine processing method on the contents of emodin and rhein in Rhizoma Polygoni Cuspidati. MethodsThe determinations of emodin and rhein were carried out by HPLC. ResultsThe emodin content in Rhizoma Polygoni Cuspidati changed as follows: sample baked with wet wine > sample baked with boiled wine>sample baked with fried wine. ConclusionThe different wine processing methods and temperature have effects on the contents of emodin of Rhizoma Polygoni Cuspidati.
Key words:Rhizoma Polygoni Cuspidati; Processing method; Emodin; Rhein; HPLC
虎杖为常用中药,来源于蓼科植物虎杖Polygonum cuspidatum .( Makino, Reynoutria japonica Houtt.)的干燥根茎及根。其性微苦,微寒。归肝、胆、肺经。具有祛风利湿,散淤定痛,止咳化痰等功效。用于治疗关节痹痛,湿热黄疸,经闭,癥瘕,水火烫伤,跌扑损伤,痈肿疮毒,咳嗽痰多等症[1]。虎杖的酒制法最早见于宋
·《太平圣惠方》:“月水不利。虎杖三两,凌霄花、没药一两为末,热酒每服一钱。又方:治二斗,水一觚,浸虎杖一宿,煎取二斗,入二汁,同煎如铴。每酒服一合……”[2]。明代仍沿用前人的经验,李时珍编撰《本草纲目》曰:“治产后淤血血痛,及坠扑昏闷:虎杖根,酒研末服[3]”。现代酒制记载有“取虎杖根,细剉,以酒浸泡5~7 日,去渣备用”[4]。侗族酒制法则为:取净虎杖用黄酒40%浸泡30 min,用文火煮至酒被吸尽,取出,切片,干燥[5]。笔者曾对虎杖不同炮制品中主要成分大黄素进行分析,发现同等条件下酒制品中大黄素的含量略高[7],现采用高效液相色谱法对虎杖酒制品中大黄素及大黄酸进行测定,以探讨其酒制品质量。
1 仪器与试剂
1100高效液相色谱仪(美国安捷伦公司);LG16-W高速离心机(北京医用离心机厂);SB32002-T超声器(Shanghaibranson);对照品大黄素、大黄酸由中国药品制品检定所提供(批号分别为