基因组学与应用生物学,2010年,第29卷,第6期,第10191025页GenomicsandAppliedBiology,2010,,,10191025AnArticle研究论文CloningandBioinformaticAnalysisofPotatoαAmylaseGeneamyA1WangYuliWangYonggangMaJianzhong*WangQianTaoPengfeiSuYiSchoolofLifeScienceandEngineering,LanzhouUniversityofTechnology,Lanzhou,730050*Correspondingauthor,******@AbstractInthisresearch,weamplifiedacDNAforpotatoαamylasebyRT--ysisshowedthatthecDNAhada1224bpopenreadingframeandwasreferredtoasamyA1,whichencodesforanαamylasewith407aminoacidresidues(essionnumber:)-quantitativeRT-,includingitscodonusagebias,physicalandchemicalproperties,subcellu-larlocalization,%identitywithapublishedpotatoαamylase(essionnumber:)(PF00128、SM00624)between20to348andaC-terminalbeta-sheetdomain(PF07821)between349~407,-strandedalpha/betabarrelwasalsofoundintheenzyme,,thetwogenesfrompotatopresentsmoreclosehomologytothosefromcassavaanda
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