芪龙胶囊的质量标准研究.doc

芪龙胶囊的质量标准研究叶素艳1,振1,周海洋1,敏1,瑞琛2,许丽丽3(,272000;,,250101)摘要:目的:修订芪龙胶囊的质量标准。方法:采用薄层色谱法对方中丹参、赤芍进行鉴别,采用高效液相色谱法测定毛蕊异黄酮葡萄糖苷的含量。色谱柱为C18(250mm×,5µm),以乙腈为流动相A,%甲酸溶液为流动相B,进行梯度洗脱;检测波长为260nm,柱温30℃,。结果:TLC鉴别专属性强,分离度好;毛蕊异黄酮葡萄糖苷在浓度25~75µg/ml围与峰面积呈良好的线性关系。%。结论:本方法简便,灵敏,准确,快速,专属性强,重现性好等优点,适合于该药的质量分析检验。关键词:芪龙胶囊;质量标准;丹参;赤芍;毛蕊异黄酮葡萄糖苷QualitystandardofQilongCapsuleYESu-yan1,LIZhen1,ZHOUHai-yang1,XIEMing1,LIURui-chen2,XULi-li3(,Jining272000,China;,ShandongUniversity,Jinan250000,China;,Jinan250101,China)ABSTRACT:OBJECTIVE::UsingTLCmethodtoidentifytheRadixSalviaeMiltiorrhizaeandRadixPaeoniaeRubra,andthecontentofCalycosin-7-(250mm×,5µm),%radientelution,thedetectionwavelengthis260nm,thecolumntemperatureis30℃,:ThespecificityofTLCidentificationisstrong,-7-glucosidehasagoodlinearrelationshipinthe25~75µg/mlrangeofconcentration,%.CONCLUSION:Theadvantagesofthismethodweresimple,sensitive,accurateandrapid,withagoodspecificityandreproducible,soit':Qi